Improved and Rapid High-Performance Liquid Chromatographic Assay for 13-cis- Retinoic Acid or All-trans-retinoic Acid

A rapid, specific, and sensitive reversed-phase high-performance liquid chromatographic (HPLC) assay for the quantitative determination of all-trans- retinoic acid (I) or 13-cis-retinoic acid (II) in rat serum without extraction or lyophilization is described. Chromatographic separation from retinol...

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Bibliographic Details
Published in:Journal of pharmaceutical sciences 1982-02, Vol.71 (2), p.262-264
Main Authors: Shelley, Rickey, Price, J.C., Jun, H.Won, Cadwallader, D.E., Capomacchia, A.C.
Format: Article
Language:English
Subjects:
13-cis-Retinoic acid—high-performance liquid chromatographic analysis
All-trans-retinoic acid-high-performance liquid chromatographic analysis
Animals
Chromatography, High Pressure Liquid - methods
D 13-cis-Retinoic acid-high-performance liquid chromatographic analysis
Half-Life
High-performance liquid chromatography-assay for 13-cis-retinoic acid and all-trans-retinoic acid
Male
Molecular Conformation
Rats
Time Factors
Tretinoin - analysis
Tretinoin - blood
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Description
Summary:A rapid, specific, and sensitive reversed-phase high-performance liquid chromatographic (HPLC) assay for the quantitative determination of all-trans- retinoic acid (I) or 13-cis-retinoic acid (II) in rat serum without extraction or lyophilization is described. Chromatographic separation from retinol, serum components, and retinol acetate standard was achieved on octadecylsilane-coated particles with aceto-nitrile-1% ammonium acetate as the eluent. Serum samples (100 μl) containing as little as 10 ng of retinoid were analyzed. Serum level profiles of rats dosed with the retinoids demonstrated the utility of the assay and indicated elimination half-lives of 0.58 and 0.92 hr for I and II, respectively.
ISSN:0022-3549
1520-6017
DOI:10.1002/jps.2600710230