New Attenuation Marker for Junin Virus Based on Immunologic Responses of Guinea Pigs

A new attenuation marker to distinguish a virulent strain (XJJV) from an attenuated strain (XJCI3JV or XJOJV) of Junin virus by means of the humoral and cellular responses to unrelated antigens was studied in guinea pigs. Strain XJJV suppressed the humoral immune response, as shown by the lower tite...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of infectious diseases 1982-03, Vol.145 (3), p.331-336
Main Authors: Galassi, Nora V., Blejer, Jorgelina L., Barrios, Hebe, Nejamkis, Marta R., Nota, Nora R.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies - analysis
Antibody Formation
Antigens
Arenaviridae - physiology
Arenaviruses, New World - physiology
Arthus reaction
Arthus Reaction - immunology
Dermatitis, Contact - immunology
Dinitrofluorobenzene
Female
Guinea Pigs
Immunity, Cellular
Infections
Junin virus
Male
Neutralizing antibodies
Ovalbumin - immunology
Tuberculin
Tuberculin Test
Vaccination
Vaccines
Vaccines, Attenuated - analysis
Virulence
Viruses
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A new attenuation marker to distinguish a virulent strain (XJJV) from an attenuated strain (XJCI3JV or XJOJV) of Junin virus by means of the humoral and cellular responses to unrelated antigens was studied in guinea pigs. Strain XJJV suppressed the humoral immune response, as shown by the lower titers of precipitating antibody to ovalbumin. The concomitant decrease in serum complement level contributed to a milder Arthus cutaneous reactivity. In contrast, the attenuated strains did not decrease the humoral response. The pathogenic strain suppressed cell-mediated immunity, as demonstrated by decreased contact sensitivity to 2,4-dinitro-l-fluorobenzene and by depression of delayed skin reactions to tuberculin purified protein derivative. When attenuated strains were used, such suppressive effects were not observed. For virulent strain XJJV, virus replication in lymphoid organs and immunosuppressive effects were correlated. These findings provide a further means to differentiate between virulent and attenuated strains of Junin virus for the purpose of vaccine control of Argentine hemorrhagic fever.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/145.3.331