Amino acid sequence of beta-galactosidase. VII. Isolation of the 24 cyanogen bromide peptides

All of the 24 cyanogen bromide peptides of beta-galactosidase have been isolated in pure form. Of these 8 ranged in size from 2 to 5 residues and were purified by paper electrophoresis. The 16 large peptides, from 23 to 119 residues, were chromatographed at pH 5.0 on a carboxymethyl-cellulose column...

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Bibliographic Details
Published in:The Journal of biological chemistry 1978-08, Vol.253 (15), p.5499-5504
Main Author: Fowler, A V
Format: Article
Language:English
Subjects:
Amino Acid Sequence
beta-Galactosidase
Cyanogen Bromide
Escherichia coli - enzymology
Galactosidases
Peptide Fragments - analysis
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Summary:All of the 24 cyanogen bromide peptides of beta-galactosidase have been isolated in pure form. Of these 8 ranged in size from 2 to 5 residues and were purified by paper electrophoresis. The 16 large peptides, from 23 to 119 residues, were chromatographed at pH 5.0 on a carboxymethyl-cellulose column in 0.02 M ammonium acetate buffer containing 8 M urea. A number of peptides were obtained in pure from following Sephadex G-50 or G-75 gel filtration. Others were separated on sulfopropyl-Sephadex or diethyl-(2-hydroxylpropylaminoethyl)-Sephadex. There large peptides were obtained in over 50% yield and several others were obtained in more than 25% yield.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(17)30401-5