Determination of the free-energy coupling for binding of calcium ions and troponin I to calmodulin

• Published in: Biochemistry (Easton) 1982-01, Vol.21 (1), p.156-162
• Main Authors: Keller, C H, Olwin, B B, LaPorte, D C, Storm, D R
• Format: Article
• Language: English
• Subjects: calcium; Calcium - metabolism; Calcium-Binding Proteins - metabolism; calmodulin; Calmodulin - metabolism; Chromatography, Gel; kinetics; Magnesium - pharmacology; Magnesium Chloride; Muscle Proteins - metabolism; Osmolar Concentration; Potassium Chloride; Protein Binding - drug effects; Spectrometry, Fluorescence; Thermodynamics; Troponin - metabolism; Troponin I
• ISSN: 0006-2960
• DOI: 10.1021/bi00530a027

Regulation of a wide variety of biological systems by Ca2+ is now known to be mediated through calmodulin, a Ca2+-binding protein. Calmodulin forms Ca2+-dependent complexes with several proteins, including troponin I. We have determined the free-energy coupling (delta GoCT) for binding of Ca2+ and troponin I to calmodulin by measuring Ca2+ binding to calmodulin and to the 1:1 calmodulin-troponin I complex by equilibrium dialysis. The dissociation constant for the Ca2+4.calmodulin and troponin I complex was also determined directly by monitoring fluorescence intensity changes accompanying complex formation between Ca2+4.CaM and N-dansylaziridine-troponin I, a fluorescent derivative of troponin I. Calmodulin displayed four Ca2+-binding sites of similar affinity with a geometric mean dissociation constant of 14 microM. In the presence of saturating troponin I, the geometric mean dissociation constant for the four Ca2+-binding sites was shifted to 1.7 microM. delta GoCT was therefore -1.25 kcal/mol of Ca2+. Saturation of calmodulin with Ca2+ would therefore be expected to increase its affinity for troponin I about 4500-fold. A dissociation constant of 20 nM was determined for the Ca2+4.calmodulin.N-dansylaziridine-troponin I complex. The dissociation constant of the calmodulin.N-dansylaziridine-troponin I complex in the absence of Ca2+ should therefore be about 90 microM. A prediction of this data is that binding of Ca2+ to calmodulin will show strong positive cooperativity when Ca2+ binding to a substoichiometric number of sites is sufficient to promote calmodulin.troponin I complex formation.