SEMIAUTOMATED TURBIDIMETRIC MICROBIOLOGICAL ASSAY FOR CEFAZAFLUR

A quantitative semi-automated turbidimetric bioassay for cefazaflur, using Streptococcus faecium as the indicator, is described. Assays were run at pH 6.5-7 for 3.75 hours at 37°C using 2-12 μg cefazaflur per ml assay broth for standards. The dose response line was plotted point to point using the n...

Full description

Saved in:
Bibliographic Details
Published in:Journal of antibiotics 1978, Vol.31(4), pp.359-362
Main Authors: PITKIN, DONALD H., ACTOR, PAUL, BALDINUS, JOSEPH G., POST, ALEX, WEISBACH, JERRY A.
Format: Article
Language:English
Subjects:
Biological Assay - methods
Cephalosporins - analysis
Hydrogen-Ion Concentration
Nephelometry and Turbidimetry
Time Factors
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A quantitative semi-automated turbidimetric bioassay for cefazaflur, using Streptococcus faecium as the indicator, is described. Assays were run at pH 6.5-7 for 3.75 hours at 37°C using 2-12 μg cefazaflur per ml assay broth for standards. The dose response line was plotted point to point using the natural log of the absorbance vs natural log of the concentration. This assay is both accurate and precise and is more rapid than traditional plate assays for antibiotics.
ISSN:0021-8820
1881-1469
DOI:10.7164/antibiotics.31.359