Immunogenic antigens common to Plasmodium knowlesi and Plasmodium falciparum are expressed on the surface of infected erythrocytes

Sera of Gambian individuals and rhesus monkeys immune against infections with Plasmodium falciparum and Plasmodium knowlesi, respectively, were reacted with Triton X-100-solubilized membranes of infected erythrocytes. Indirect immune precipitation with Staphylococcus aureus, Cowan strain A, followed...

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Bibliographic Details
Published in:The Journal of parasitology 1982-04, Vol.68 (2), p.185-193
Main Authors: Schmidt-Ullrich, R, Miller, L H, Wallach, D F, Lightholder, J
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibody Formation
Antigens
Antigens - immunology
Antiserum
Cell membranes
Erythrocyte membrane
Erythrocyte Membrane - immunology
Erythrocytes
Erythrocytes - immunology
Humans
Immunology
Macaca mulatta
Malaria
Malaria - immunology
Molecular Weight
P branes
Parasites
Parasitism
Plasmodium - immunology
Plasmodium falciparum - immunology
Species Specificity
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Summary:Sera of Gambian individuals and rhesus monkeys immune against infections with Plasmodium falciparum and Plasmodium knowlesi, respectively, were reacted with Triton X-100-solubilized membranes of infected erythrocytes. Indirect immune precipitation with Staphylococcus aureus, Cowan strain A, followed by dodecylsulfate-polyacrylamide gel electrophoresis, were used to identify interspecies plasmodial antigens that were immunogenic in vivo. Both types of sera specifically precipitated Plasmodium-specific antigens with MrS of 125,000, 90,000, and 65,000 to 50,000 from membranes of P. knowlesi-infected erythrocytes that had been labeled with 125I using the lactoperoxidase-catalyzed radioiodination or metabolically with 14C-amino acids. In addition, P. falciparum inhibited the precipitation of P. knowlesi antigens by the Gambian immune sera. Our results indicate, that during erythrocytic schizogony, interspecies Plasmodium antigens are exposed on the surfaces of infected erythrocytes.
ISSN:0022-3395
1937-2345
DOI:10.2307/3281171