ISOLATION AND CHARACTERIZATION OF TWO 16-MEMBERED LACTONES: 20-DEOXOROSARAMICIN AND 20-DEOXO-12, 13-DESEPOXY-12, 13-DEHYDROROSARAMICIN AGLYCONES, FROM A MUTANT STRAIN OF MICROMONOSPORA ROSARIA

Rosaramicin, a 16-membered ring macrolide antibiotic, was isolated from the culture filtrate of Micromonospora rosaria . The biosynthesis of rosaramicin was previously investigated and established the incorporation of radiolabeled acetic, propionic, and butyric acid and L-methionine into rosaramicin...

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Bibliographic Details
Published in:Journal of antibiotics 1982, Vol.35(2), pp.251-253
Main Authors: VAUGHAN, RICHARD W., LOTVIN, JASON, PUAR, MOHINDAR S., PATEL, MAHESH, KERSHNER, ALLAN, KALYANPUR, M. G., MARQUEZ, JOSEPH, WAITZ, J. ALLAN
Format: Article
Language:English
Subjects:
Leucomycins - biosynthesis
Leucomycins - isolation & purification
Magnetic Resonance Spectroscopy
Mass Spectrometry
Micromonospora - analysis
Micromonospora - genetics
Mutation
Tylosin - analogs & derivatives
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Summary:Rosaramicin, a 16-membered ring macrolide antibiotic, was isolated from the culture filtrate of Micromonospora rosaria . The biosynthesis of rosaramicin was previously investigated and established the incorporation of radiolabeled acetic, propionic, and butyric acid and L-methionine into rosaramicin. The authors explored the biosynthetic route of rosaramicin using intermediates and idiotrophs using mutants blocked in rosaramicin biosynthesis. Mutant T6071 was obtained which produced a mixture of two 16-membered lactones, 20-deoxorosaranolide and 20-deoxo-12,13-desepoxy-12,13-dehydrorosaranolide. This paper describes the fermentation, isolation, and characterization of the two components.
ISSN:0021-8820
1881-1469
DOI:10.7164/antibiotics.35.251