Long-Term Tissue Cultures of Human Pleural Effusions: A Cytological Follow-Up

A cytological follow-up was performed on long-term cultures of 53 metastatic pleural effusions and 34 cases of mesothelial hyperplasia. Cells were grown in Leighton tubes with up to 11 changes of cover slip in the same tube. Cell morphology and arrangement was followed for up to 1 year. Morphologica...

Full description

Saved in:
Bibliographic Details
Published in:In Vitro 1978-07, Vol.14 (7), p.591-600
Main Authors: Mouriquand, J, Mouriquand, C, Petitpas, E, Mermet, M A
Format: Article
Language:English
Subjects:
Animal cells
Cancer
Cell Count
Cell culture techniques
Cell growth
Cell lines
Cell Nucleolus - ultrastructure
Cells, Cultured
Chromatin - ultrastructure
Cultured cells
Cytoplasm - ultrastructure
Epithelial cells
Epithelium - pathology
Humans
Hyperplasia
Hyperplasia - pathology
Neoplasm Metastasis
Neoplasms - pathology
Pleural effusion
Pleural Effusion - cytology
Time Factors
Tumor cell line
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A cytological follow-up was performed on long-term cultures of 53 metastatic pleural effusions and 34 cases of mesothelial hyperplasia. Cells were grown in Leighton tubes with up to 11 changes of cover slip in the same tube. Cell morphology and arrangement was followed for up to 1 year. Morphological criteria are of no use in recognizing malignant cells with any degree of certainty. As to cell distribution, several types of heaping up are observed both in benign and malignant pleural effusions. In malignant cases, patches of cells in an epithelial-like arrangement are seen heaping up from their substrate. They can be recovered from the used medium by cytocentrifugation. Such behavior may explain the numerous unsuccessful attempts to establish epithelial cell lines, as the clusters of epithelial cells are progressively lost in the supernates.
ISSN:0073-5655
1475-2689
DOI:10.1007/BF02617918