Demonstration of Components of Serum-Free Culture Medium Effecting Maximum In vitro Expression of Mouse Mammary Tumor Virus

By using a chemically defined serum-free (SF) medium for propagation of$Mm5mtc_1$mouse adenocarcinoma cell cultures and clonal derivatives, medium components including hormones, glucose and individual amino acids were evaluated as to modulation of mouse mammary tumor virus (MMTV) production. Insulin...

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Bibliographic Details
Published in:In Vitro 1978-02, Vol.14 (2), p.218-226
Main Authors: Nagle, S C, Fine, D L
Format: Article
Language:English
Subjects:
Amino acids
Amino Acids - pharmacology
Animals
Cancer
Cell culture techniques
Cell growth
Cell Line
Culture Media
Dexamethasone - pharmacology
Glucose - pharmacology
Hormones
Hydrocortisone - pharmacology
Insulin
Insulin - pharmacology
Mammary Tumor Virus, Mouse - drug effects
Mammary Tumor Virus, Mouse - growth & development
Mice
Mice, Inbred C3H
Mouse mammary tumor virus
Oncogenic viruses
RNA-Directed DNA Polymerase - analysis
Tumors
Virus Cultivation
Viruses
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Summary:By using a chemically defined serum-free (SF) medium for propagation of$Mm5mtc_1$mouse adenocarcinoma cell cultures and clonal derivatives, medium components including hormones, glucose and individual amino acids were evaluated as to modulation of mouse mammary tumor virus (MMTV) production. Insulin, hydrocortisone and dexamethasone each increased MMTV production on a per cell basis over constitutive expression that occurs in SF medium devoid of hormones. Maximum production occurred when all three hormones were present. Hormone-stimulated virus expression also was influenced by glucose concentration. Cell growth and maximum MMTV expression increased when thyroxine, asparagine, proline and serine were omitted from the medium formulation. The resulting modified SF medium provides and ideal system for the propagation of high MMTV-producer clones and for the study of the biochemical regulation of MMTV expression.
ISSN:0073-5655
1475-2689
DOI:10.1007/BF02618226