In vitro Stimulation of Rabbit T Lymphocytes by Cells Expressing Herpes Simplex Antigens

• Published in: Journal of general virology 1982-04, Vol.59 (2), p.415-419
• Main Authors: Kapoor, A. K, Ling, N. R, Nash, A. A, Buchan, A, Wildy, P
• Format: Article
• Language: English
• Subjects: Animals; Antigens, Viral - immunology; Cell Line; Cricetinae; herpes simplex virus; Lymphocyte Activation; Mutation; Rabbits; Simplexvirus - growth & development; Simplexvirus - immunology; T-Lymphocytes - immunology; Temperature
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-59-2-415

Departments of Experimental Pathology and Virology The Medical School, Birmingham B15 2TJ, U.K. Lymphocyte stimulation responses to herpes antigens were studied using virus-infected X-irradiated cells. Rabbits were immunized with herpes simplex virus type 1 (strain HFEM) grown in RK13 cells. For in vitro stimulation assay BHK21 cells were X-irradiated (15000 rad) and infected with a high m.o.i. of a temperature-sensitive ( ts ) mutant (N102) of HFEM strain at the non-permissive temperature (38.5 °C) of virus. Virus antigens were expressed on the infected cells and there was no leakage of infectious virus into the medium at 38.5 °C. T lymphocytes from rabbits immunized with herpes simplex virus were specifically activated by herpesvirus-infected X-irradiated cells; lymph node cells from rabbits immunized with RK13 cells and from non-immune rabbits showed no proliferative response. Keywords: HSV, rabbit, T cell stimulation, infected cells Present address: Department of Pathology, K.G.'s Medical College, Lucknow, U.P., India. Present address: Department of Immunology, The Medical School, Birmingham B15 2TJ, U.K. Present address: Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, U.K. || Present address: Department of Medical Microbiology, The Medical School, Birmingham B15 2TJ, U.K. Received 2 October 1981; accepted 14 December 1981.