Histological and Cytological Technique for the Quantitation of Cultured Human Bone-Marrow Cells: Formation of Aggregates

A bone-marrow culture system is described that provides a simple, quantitative and rapid assessment of marrow bone cells in vitro. Aggregation of bone-marrow cells, an in vitro phenomenon, occurs within 24 hr of culture and is observed utilizing Millipore filters. Daily quantitation shows both an in...

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Bibliographic Details
Published in:In Vitro 1978-06, Vol.14 (6), p.495-501
Main Authors: Garry, Vincent F., Nesbitt, Mark, White, James, Gerald Vosika
Format: Article
Language:English
Subjects:
B lymphocytes
Bone marrow
Bone Marrow Cells
Cell Adhesion
Cell aggregates
Cell Aggregation
Cell culture techniques
Cell Survival
Cells, Cultured
Cultured cells
Fibroblasts - cytology
Granulocytes - cytology
Hematopoietic stem cells
Humans
Lymphocytes
Macrophages - cytology
Myeloid cells
Neutrophils
Neutrophils - cytology
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Summary:A bone-marrow culture system is described that provides a simple, quantitative and rapid assessment of marrow bone cells in vitro. Aggregation of bone-marrow cells, an in vitro phenomenon, occurs within 24 hr of culture and is observed utilizing Millipore filters. Daily quantitation shows both an increase in the number and a change in the morphology of these aggregates. The maximum number of aggregates is achieved on the 2nd or 3rd day of incubation. Histologically, aggregates are composed of myeloid, mononuclear and mesenchymal fibroblastic cells. Mesenchymal cells form a matrix for apposed mononuclear and myeloid cells. Scanning electron micrographs show intimate cell contact and spreading by the marrow cells. Fluctuation of the absolute numbers of various cell types are observed. The system can be utilized for long-term culture of bone marrow.
ISSN:0073-5655
1475-2689
DOI:10.1007/BF02616089