The release of serotonin from rat duodenal enterochromaffin cells by adrenoceptor agonists studied in vitro

The serotonin (5‐HT) content of enterochromaffin cells (EC) was studied by a cytofluorimetric method in biopsies from rat duodenal mucosa after in vitro incubation with different adrenoceptor agonists and antagonists and acetylcholine (ACh). Noradrenaline (NA), Adrenaline (A) and Isoprenaline (IP) c...

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Bibliographic Details
Published in:Acta physiologica Scandinavica 1978-06, Vol.103 (2), p.219-224
Main Authors: Pettersson, Gösta, Dahlström, Annica, Larsson, Inger, Lundberg, Jan M., Ahlman, Häkan, Kewenter, Jan
Format: Article
Language:English
Subjects:
Acetylcholine - pharmacology
Adrenergic beta-Agonists
Adrenergic beta-Antagonists
Animals
Chromaffin System
cytofluorimetry
Dopamine - pharmacology
duodenal mucosa
Duodenum - metabolism
Enterochromaffin Cells
Epinephrine - pharmacology
in vitro
Isoproterenol - pharmacology
Male
Metoprolol - pharmacology
Norepinephrine - pharmacology
Phenoxybenzamine - pharmacology
Phentolamine - pharmacology
Propranolol - pharmacology
Rat enterochromaffin cells
Rats
Serotonin - secretion
serotonin release
β-adrenoceptor
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Summary:The serotonin (5‐HT) content of enterochromaffin cells (EC) was studied by a cytofluorimetric method in biopsies from rat duodenal mucosa after in vitro incubation with different adrenoceptor agonists and antagonists and acetylcholine (ACh). Noradrenaline (NA), Adrenaline (A) and Isoprenaline (IP) caused a decrease of 5‐HT in EC down to 40–60% and for NA and A this effect was concentration‐dependent. The effect was antagonized by d,1‐propranolol but not by d‐propranolol, metoprolol, phentolamine or phenoxy‐benzamine indicating that the 5‐HT release from EC is probably mediated via a true β‐adrenoceptor mechanism possibly of the β2 type. ACh also decreased the 5‐HT content of EC but was much less potent than the adrenergic substances. Dopamine (DA) had no effect.
ISSN:0001-6772
1365-201X
DOI:10.1111/j.1748-1716.1978.tb06209.x