Acetylation of chromosome squashes of Drosophila melanogaster decreases the background in autoradiographs from hybridization with (125I) labeled RNA

DNA in prepared chromosomes from the larval salivary glands of Drosophila melanogaster was hybridized with [125I]-labeled 5S and tRNA from the same organism. Autoradiography revealed that radioactivity was frequently bound to all regions of the slides, masking labeling of the chromosomes. Acetylatio...

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Bibliographic Details
Published in:The journal of histochemistry and cytochemistry 1978-08, Vol.26 (8), p.677-679
Main Authors: Hayashi, S, Gillam, I.C, Delaney, A.D, Tener, G.M
Format: Article
Language:English
Subjects:
Acetylation
Animals
arthropods
Autoradiography
Chromosomes - analysis
DNA - analysis
Drosophila melanogaster - genetics
entomology
Genes
insects
iodine
Iodine Radioisotopes
isotopes
Larva
Mutation
Nucleic Acid Hybridization
RNA
RNA, Transfer
Salivary Glands - ultrastructure
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Description
Summary:DNA in prepared chromosomes from the larval salivary glands of Drosophila melanogaster was hybridized with [125I]-labeled 5S and tRNA from the same organism. Autoradiography revealed that radioactivity was frequently bound to all regions of the slides, masking labeling of the chromosomes. Acetylation of the preparations before hybridization prevented the formation of this background and revealed the specific chromosomal sites.
ISSN:0022-1554
1551-5044
DOI:10.1177/26.8.99471