G6PD in Immature and Mature Human Brain: Electrophoretic and Enzyme Kinetic Studies

The isoenzyme polymorphism and kinetics of glucose-6-phosphate dehydrogenase (G6PD) in embryonal and mature human brain were studied. Two types of electropherograms were found in embryonal brains: type I is characterized by two G6PD fractions: slow and fast, and in the type II a single, fast-moving...

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Bibliographic Details
Published in:Human heredity 1982-01, Vol.32 (3), p.193-196
Main Authors: Toncheva, D., Evrev, T., Tzoneva, M.
Format: Article
Language:English
Subjects:
Brain - embryology
Brain - enzymology
Chromosome Mapping
Electrophoresis, Polyacrylamide Gel
Genetic Variation
Glucosephosphate Dehydrogenase - genetics
Glucosephosphate Dehydrogenase - metabolism
Humans
Isoenzymes - genetics
Isoenzymes - metabolism
Kinetics
Original Papers
Polymorphism, Genetic
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Summary:The isoenzyme polymorphism and kinetics of glucose-6-phosphate dehydrogenase (G6PD) in embryonal and mature human brain were studied. Two types of electropherograms were found in embryonal brains: type I is characterized by two G6PD fractions: slow and fast, and in the type II a single, fast-moving G6PD fraction was seen in a single embryo. The additional studies carried out, namely reelectrophoresis following 2-mercaptoethanol and NADP treatment and the specific enzyme kinetics, i. e. altered Michaelis constant and enhanced utilization of the desamino-NADP analogue which are typical of the fast-moving fraction in embryonal brain, speak unequivocally in favor of the presence of a new, unknown, phase-specific isoenzyme of G6PD. Its synthesis takes place only during embryonal brain development. A probable autosomal localization for the gene of brain G6PD is suggested.
ISSN:0001-5652
1423-0062
DOI:10.1159/000153290