The importance of cell contact for the proliferation of neuroblasts in culture and its stimulation by meningeal extract

The influence of cell density and cell contacts on the proliferation of neuroblasts in culture and its stimulation by meningeal extract were investigated. Dissociated brain cells from 6-day-old chick embryos were cultured under 3 different culture conditions to obtain dense or sparse brain cell cult...

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Bibliographic Details
Published in:Neurochemical research 1982-03, Vol.7 (3), p.287-300
Main Authors: Barakat, I, Sensenbrenner, M, Vincendon, G
Format: Article
Language:English
Subjects:
Animals
Cell Communication
Cell Count
Cell Division - drug effects
Cells, Cultured
Chick Embryo
Collagen
DNA - biosynthesis
Meninges - physiology
Neurons - cytology
Neurons - metabolism
Polylysine
Thymidine - metabolism
Tissue Extracts - pharmacology
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Summary:The influence of cell density and cell contacts on the proliferation of neuroblasts in culture and its stimulation by meningeal extract were investigated. Dissociated brain cells from 6-day-old chick embryos were cultured under 3 different culture conditions to obtain dense or sparse brain cell cultures, as well as cultures of isolated neuronal cell. The proliferation fo neuroblasts, shown by morphological observations, cell counts, determinations of DNA content and measurements of [3H]thymidine incorporation, was found to be the highest in cultures where cell density and cellular contacts were greatest. The addition of meningeal extract stimulated the multiplication of neuroblasts only in cultures where the cells were in closer with each other. The results suggested, therefore, that cell density and cell-cell interactions are of importance and favored neuroblast proliferation.
ISSN:0364-3190
1573-6903
DOI:10.1007/BF00965641