Fluorescence Microscopy: Reduced Photobleaching of Rhodamine and Fluorescein Protein Conjugates by n-Propyl Gallate

n-Propyl gallate (0.1 to 0.25 molar, in glycerol) reduces by a factor of 10 the rate of fading of fluorescence of cell structures labeled with tetramethylrhodamine or fluorescein-conjugated antibodies. Hence, prolonged photographic exposure of immunofluorescently labeled cells in the fluorescence mi...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1982-09, Vol.217 (4566), p.1252-1255
Main Authors: Giloh, Haim, Sedat, John W.
Format: Article
Language:English
Subjects:
Antibodies
Cell lines
Cell nucleus
Cells
Dyes
Fluorescein
Fluoresceins
Fluorescence
Free Radicals
Gallates
Gallic Acid - analogs & derivatives
Microscopy
Microscopy, Fluorescence - methods
Photochemistry
Propyl Gallate
Rhodamines
Spleen
Xanthenes
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Summary:n-Propyl gallate (0.1 to 0.25 molar, in glycerol) reduces by a factor of 10 the rate of fading of fluorescence of cell structures labeled with tetramethylrhodamine or fluorescein-conjugated antibodies. Hence, prolonged photographic exposure of immunofluorescently labeled cells in the fluorescence microscope yields images with increased sensitivity, making feasible multiple data collection, as with serial optical sectioning.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.7112126