microtiter test for detecting and titrating noncytopathogenic bovine viral diarrhea virus

Bovine cells free of noncytopathogenic bovine viral diarrhea virus (NC-BVDV) treated with polyriboinosinic acid : polyribocytidylic acid (poly I:C) were protected against challenge with vesicular stomatitis virus (VSV), whereas NC-BVDV-infected cells treated with poly I:C were not protected against...

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Bibliographic Details
Published in:Archives of virology 1982, Vol.72 (4), p.279-287
Main Authors: Maisonnave, J, Rossi, C.R
Format: Article
Language:English
Subjects:
animal diseases
animal health
Animals
Antibodies, Viral - analysis
Cattle
Cell Line
Cytopathogenic Effect, Viral
Diarrhea Viruses, Bovine Viral - growth & development
Diarrhea Viruses, Bovine Viral - immunology
Microbiological Techniques
Pestivirus - growth & development
Poly I-C - pharmacology
Time Factors
Vesicular stomatitis Indiana virus - growth & development
viral diseases of animals and humans
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Summary:Bovine cells free of noncytopathogenic bovine viral diarrhea virus (NC-BVDV) treated with polyriboinosinic acid : polyribocytidylic acid (poly I:C) were protected against challenge with vesicular stomatitis virus (VSV), whereas NC-BVDV-infected cells treated with poly I:C were not protected against VSV. An assay based on the ability of NC-BVDV to inhibit poly I:C protection of cells against VSV was developed and is herein referred to as PINBA (poly I:C for NC-BVDV assay). Noncytopathogenic BVDV was titrated as cytopathogenic strains except that several days after infection with NC-BVDV, the cultures were treated with poly I:C and VSV. Titration endpoints were reached 24 hours later. PINBA was standardized for amount of VSV, time of addition of poly I:C, and time NC-BVDV had to be present to obtain stable titration endpoints. PINBA also was useful for titrating virus neutralizing antibodies. Compared with the fluorescent antibody test, PINBA was less subjective for detection of NC-BVDV. Compared with the interference test in which NC-BVDV infected cultures are challenged with a cytopathogenic strain of BVDV, PINBA was more reliable. The technique described herein is a simple and practical microtiter method for titrating NC-BVDV and virus neutralizing antibodies and for the presumptive detection of NC-BVDV.
ISSN:0304-8608
1432-8798
DOI:10.1007/BF01315224