Preferential Association of Newly Synthesized H3 and H4 Histones with Newly Replicated DNA

The assembly of newly synthesized histones into chromatin during replication of MH-134SC cells was studied. Cells pulse-labeled with iododeoxyuridine and [3H]lysine were mixed with an equivalent number of normal cells labeled with [14C]lysine. Nuclease chromatin obtained from pooled cells was fracti...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1978-10, Vol.84 (4), p.985-988
Main Authors: SENSHU, Tatsuo, FUKUDA, Mitsugu, OHASHI, Mochihiko
Format: Article
Language:English
Subjects:
Cell Line
Chromatin - metabolism
DNA - metabolism
DNA Replication
Histones - biosynthesis
Idoxuridine
Lysine - metabolism
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Summary:The assembly of newly synthesized histones into chromatin during replication of MH-134SC cells was studied. Cells pulse-labeled with iododeoxyuridine and [3H]lysine were mixed with an equivalent number of normal cells labeled with [14C]lysine. Nuclease chromatin obtained from pooled cells was fractionated by buoyant-density centrifugation in a gradient containing Metrizamide and 3-iodo-l,2-propanediol. Histones extracted from heavy and normal chromatin regions of the gradient were fractionated by acid-urea gel electrophoresis, and 3H/14C ratios of individual histones were compared. The results showed highly preferential association of newly synthesized H3 and H4 with newly replicated DNA.
ISSN:0021-924X
DOI:10.1093/oxfordjournals.jbchem.a132213