A combined retrograde transport and immunocytochemical staining method for demonstrating the origins of serotonergic projections

In recent years considerable interest has been directed toward defining projections of neurons containing specific neurotransmitter substances. As a result, numerous techniques have been developed that use fluorescent retrograde cell markers or horseradish peroxidase combined with the fluorescence h...

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Bibliographic Details
Published in:The journal of histochemistry and cytochemistry 1982-08, Vol.30 (8), p.805-810
Main Authors: Bowker, R M, Westlund, K N, Sullivan, M C, Coulter, J D
Format: Article
Language:English
Subjects:
Afferent Pathways - physiology
Animals
Axonal Transport
Brain Stem - physiology
Cats
Dendrites - physiology
Haplorhini
Horseradish Peroxidase
Lectins
Neurons - cytology
Neurons - physiology
Raphe Nuclei - cytology
Raphe Nuclei - physiology
Rats
Serotonin - physiology
Spinal Cord - cytology
Spinal Cord - physiology
Wheat Germ Agglutinins
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Summary:In recent years considerable interest has been directed toward defining projections of neurons containing specific neurotransmitter substances. As a result, numerous techniques have been developed that use fluorescent retrograde cell markers or horseradish peroxidase combined with the fluorescence histochemistry, immunohistofluorescence, or the unlabeled antibody, peroxidase-antiperoxidase (PAP) method to identify the projections of neurotransmitter-defined neurons. In the present article, these methods are applied to determining the origin of descending serotonergic pathways to the spinal cord,
ISSN:0022-1554
1551-5044
DOI:10.1177/30.8.6181123