Monoclonal Antibody to 5-Bromo- and 5-Iododeoxyuridine: A New Reagent for Detection of DNA Replication

Monoclonal antibodies specific for 5-bromodeoxyuridine have been produced and applied in detecting low levels of DNA replication on a cell-by-cell basis in vitro. The immunoglobulin-producing hybridomas were derived from spleen cells of mice immunized with a conjugate of iodouridine and ovalbumin. T...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1982-10, Vol.218 (4571), p.474-475
Main Author: Gratzner, Howard G.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, Monoclonal
B lymphocytes
Bromodeoxyuridine - analysis
Bromodeoxyuridine - immunology
Cells, Cultured
Cultured cells
Deoxyribose synthesis
DNA Replication
Flow Cytometry
Fluorescence
Hybridomas
Idoxuridine - analysis
Idoxuridine - immunology
Immunoglobulins
Mice
Monoclonal antibodies
Phagocytes
Placenta
Plasma cells
Plasmacytoma
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Summary:Monoclonal antibodies specific for 5-bromodeoxyuridine have been produced and applied in detecting low levels of DNA replication on a cell-by-cell basis in vitro. The immunoglobulin-producing hybridomas were derived from spleen cells of mice immunized with a conjugate of iodouridine and ovalbumin. The cells were fused with the plasmacytoma line SP2/0Ag14. The antibodies produced are highly specific for bromodeoxyuridine and iododeoxyuridine and do not cross-react with thymidine. DNA synthesis in cultured cells exposed to bromodeoxyuridine for as short a time as 6 minutes can be detected easily and rapidly by an immunofluorescent staining method and quantitated by flow cytometry.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.7123245