Induction of sister chromatid exchanges by 5-fluorodeoxycytidine: correlation with DNA methylation

Chinese hamster ovary cells grown in medium containing 10 microM 5-fluorodeoxycytidine for forty-eight hours were found to have up to 5% of the deoxycytidine residues of the DNA substituted by this analog. Cytological studies of these cells showed that the incorporated 5-FdC caused a two-fold increa...

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Bibliographic Details
Published in:Chromosoma 1982-01, Vol.85 (5), p.603-609
Main Authors: Chambers, J C, Taylor, J H
Format: Article
Language:English
Subjects:
Animals
Cell Line
Chromatids - drug effects
Cricetinae
Cricetulus
Crossing Over, Genetic
Cytosine - analogs & derivatives
DNA - metabolism
Dose-Response Relationship, Drug
Female
Flucytosine - pharmacology
Methylation
Ovary
Sister Chromatid Exchange
Time Factors
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Summary:Chinese hamster ovary cells grown in medium containing 10 microM 5-fluorodeoxycytidine for forty-eight hours were found to have up to 5% of the deoxycytidine residues of the DNA substituted by this analog. Cytological studies of these cells showed that the incorporated 5-FdC caused a two-fold increase of sister chromatid exchanges (SCE) over the control level. However, 5-FdC was capable of inducing SCE only after it had been present in the cell for at least two cycles of DNA synthesis. This is in contrast to several other chemicals that we have tested which induced SCE immediately after the first DNA synthesis. We consider the possibility that the delayed effect may be related to hypomethylation of cytosine in the newly replicated DNA.
ISSN:0009-5915
1432-0886
DOI:10.1007/BF00330774