Sex preselection in mammals? Separation of sperm bearing Y and "O" chromosomes in the vole Microtus oregoni [Relevant to sex selection in agriculturally important animals and humans]

The two sex determining sperm populations of the vole Microtus oregoni were separated according to DNA content by use of flow sorting instrumentation. Although the sperm were not viable, they should be useful for addressing the question of haploid expression of genes linked to sex chromosomes and fo...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1982-11, Vol.218 (4575), p.904-906
Main Authors: Pinkel, D, Gledhill, B.L, Lake, S, Stephenson, D, Van Dilla, M.A
Format: Article
Language:English
Subjects:
Animals
Arvicolinae - genetics
DNA - analysis
Flow Cytometry - methods
Fluorescence
Fluorescent Dyes
Histograms
Humans
Male
Mammals
Sex chromosomes
Sex Chromosomes - ultrastructure
Sex determination (Genetics)
Sex Determination Analysis
Sex determination, Genetic
Sex preselection
Somatic cells
Sperm
Spermatozoa
Spermatozoa - physiology
Visual cortex
Voles
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Summary:The two sex determining sperm populations of the vole Microtus oregoni were separated according to DNA content by use of flow sorting instrumentation. Although the sperm were not viable, they should be useful for addressing the question of haploid expression of genes linked to sex chromosomes and for efficiently searching for biochemical markers that differentiate the two populations.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.6753153