Purification and characterization of avian liver mevalonate-5-pyrophosphate decarboxylase

Mevalonate-5-pyrophosphate decarboxylase [ATP:5-diphosphomevalonate carboxy-lyase (dehydrating), EC 4.1.1.33] has been purified 5800 times from chicken liver and obtained in a stable and highly purified form. The protein is a dimer of molecular weight 85400 +/- 1941, and its subunits were not resolv...

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Published in:Biochemistry (Easton) 1982-09, Vol.21 (19), p.4646-4650
Main Authors: Alvear, Marysol, Jabalquinto, Ana Maria, Eyzaguirre, Jaime, Cardemil, Emilio
Format: Article
Language:English
Subjects:
Animals
Carboxy-Lyases - isolation & purification
Carboxy-Lyases - metabolism
Cations, Divalent
Chickens
Edetic Acid - pharmacology
Kinetics
Liver - enzymology
Molecular Weight
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container_issue 19
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container_title Biochemistry (Easton)
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creator Alvear, Marysol
Jabalquinto, Ana Maria
Eyzaguirre, Jaime
Cardemil, Emilio
description Mevalonate-5-pyrophosphate decarboxylase [ATP:5-diphosphomevalonate carboxy-lyase (dehydrating), EC 4.1.1.33] has been purified 5800 times from chicken liver and obtained in a stable and highly purified form. The protein is a dimer of molecular weight 85400 +/- 1941, and its subunits were not resolved by gel electrophoresis in denaturing conditions. The purified enzyme does not require the presence of SH-containing reagents for either activity or stability. The enzyme shows a high specificity for adenosine 5'-triphosphate (ATP) and requires for activity a divalent metal cation, Mg2+ being most effective. The optimum pH for the enzyme ranges from 4.0 to 6.5. Inhibitory effects for the enzyme activity were detected by citrate, phthalate, and phosphate. The isoelectric point, as determined by column chromatofocusing, is 4.8. The kinetics are hyperbolic for both substrates, showing a sequential mechanism; true Km values of 0.0141 mM and 0.504 mM have been obtained for mevalonate-5-pyrophosphate and ATP, respectively.
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source American Chemical Society
subjects Animals
Carboxy-Lyases - isolation & purification
Carboxy-Lyases - metabolism
Cations, Divalent
Chickens
Edetic Acid - pharmacology
Kinetics
Liver - enzymology
Molecular Weight
title Purification and characterization of avian liver mevalonate-5-pyrophosphate decarboxylase
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