Fatty acyl chain order in lecithin model membranes determined from proton magnetic resonance

Proton magnetic resonance (1H NMR) has been used to compare the local orientational order of acyl chains in phospholipid bilayers of multilamellar and small sonicated vesicular membranes of dipalmitoyllecithin (DPL) at 50 degrees C and egg yolk lecithin (EYL) at 31 degrees C. The orientational order...

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Bibliographic Details
Published in:Biochemistry (Easton) 1978-12, Vol.17 (26), p.5750-5762
Main Authors: Bloom, Myer, Burnell, E. Elliott, MacKay, Alexander L, Nichol, Christine P, Valic, Marko I, Weeks, Gerald
Format: Article
Language:English
Subjects:
Fatty Acids
Magnetic Resonance Spectroscopy
Mathematics
Membranes, Artificial
Models, Biological
Molecular Conformation
Phosphatidylcholines
Pulmonary Surfactants
Structure-Activity Relationship
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Summary:Proton magnetic resonance (1H NMR) has been used to compare the local orientational order of acyl chains in phospholipid bilayers of multilamellar and small sonicated vesicular membranes of dipalmitoyllecithin (DPL) at 50 degrees C and egg yolk lecithin (EYL) at 31 degrees C. The orientational order of the multilamellar systems was characterized using deuterium magnetic resonance order parameters and 1H NMR second moments. 1H NMR line shapes in the vesicle samples were calculated using vesicle size distributions, determined directly using electron microscopy, and a theory of motional narrowing, which takes into account the symmetry properties of the bilayer systems. The predicted non-Lorentzian line shapes and widths were found to be in good agreement with experimental results, indicating that the local orientational order (called "packing" by many workers) in the bilayers of small vesicles and in multilamellar membranes is substantially the same. This results was found to be true not only for the largest 1H NMR line associated with the nonterminal methylene protons but also for the resolved 1H NMR lines due to the alpha-CH2 and the terminal CH3 positions on the acyl chain. Analysis of the vesicle 1H NMR spectra of EYL taken with different medium viscosities yielded a value of approximately 4 X 10(-8) cm2 s-1 for the lateral diffusion constant of the phospholipid molecules at 31 degrees C.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi00619a024