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Immunoglobulin isotype expression of normal pre-B cells as determined by immunofluorescence

We have undertaken an immunofluorescent analysis of the immunoglobulin heavy and light chains expressed by pre-B cells from normal human fetal and adult bone marrow using purified mouse monoclonal and goat antibodies to human immunoglobulin isotypes. Our results indicate that (i) the great majority...

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Published in:	Journal of clinical immunology 1982-10, Vol.2 (4), p.264-269
Main Authors:	Kubagawa, H, Gathings, W E, Levitt, D, Kearney, J F, Cooper, M D
Format:	Article
Language:	English
Subjects:	Adult B-Lymphocytes - immunology Bone Marrow - immunology Cell Differentiation Fetus - immunology Fluorescent Antibody Technique Humans Immunoglobulin Allotypes Immunoglobulin Heavy Chains Immunoglobulin Light Chains Immunoglobulin mu-Chains Leukemia, Lymphoid - immunology
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description	We have undertaken an immunofluorescent analysis of the immunoglobulin heavy and light chains expressed by pre-B cells from normal human fetal and adult bone marrow using purified mouse monoclonal and goat antibodies to human immunoglobulin isotypes. Our results indicate that (i) the great majority of normal pre-B cells in both fetuses and adults expresses intracytoplasmic mu chains only, (ii) immunoglobulin light-chain synthesis may be initiated during a late stage in pre-B-cell development, and (iii) heavy-chain isotype switching at the pre-B-cell stage may not occur during normal B-cell development.
doi_str_mv	10.1007/BF00915065
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subjects	Adult B-Lymphocytes - immunology Bone Marrow - immunology Cell Differentiation Fetus - immunology Fluorescent Antibody Technique Humans Immunoglobulin Allotypes Immunoglobulin Heavy Chains Immunoglobulin Light Chains Immunoglobulin mu-Chains Leukemia, Lymphoid - immunology
title	Immunoglobulin isotype expression of normal pre-B cells as determined by immunofluorescence
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