Polydispersity and chain break effects on dna intramolecular renaturation

The influences of sample polydispersity and of internal chain breaks are introduced in the formal description of intramolecular renaturation phenomenology, enhancing the usefulness of the involved methodology. In our method, renaturation is induced not by cooling but by increasing the sodium concent...

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Bibliographic Details
Published in:Biophysical chemistry 1982-01, Vol.16 (1), p.61-66
Main Authors: Mingot, Francisco, Acun̄a, M.Isabel, Garces, Fernando, DaVila, Carlos A.
Format: Article
Language:English
Subjects:
Animals
Cattle
DNA
Kinetics
Mathematics
Molecular Weight
Nucleic Acid Denaturation
Nucleic Acid Renaturation
Polydispersity
Renaturation
Temperature
Thymus Gland
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Summary:The influences of sample polydispersity and of internal chain breaks are introduced in the formal description of intramolecular renaturation phenomenology, enhancing the usefulness of the involved methodology. In our method, renaturation is induced not by cooling but by increasing the sodium concentration. The single-stranded size distributions for the two types of samples employed (haploiomic and diplotomically degraded) are discussed. In the case of haplotomic cleavage, the variable is the strand size in the ‘segment’ between consecutive nicks in either of the native DNA strands. Our equations have been obtained by considering that the arrangement of DNA sequences may be approximately taken as random. These equations provide a good description of experimental data and a reasonable value (about 1000 base-pairs) for the size of the thermahte sequence, but show low sensitivity to departures from the random arrangement of sequences.
ISSN:0301-4622
1873-4200
DOI:10.1016/0301-4622(82)85008-4