Genetic and Antigenic Variation in Type 3 Polioviruses: Characterization of Strains by Monoclonal Antibodies and T1 Oligonucleotide Mapping

National Institute for Biological Standards and Control, Holly Hill, Hampstead, London NW3 6RB, U.K. Considerable genetic and antigenic heterogeneity was detected among a collection of 17 poliovirus type 3 strains isolated between 1939 and 1958 in studies using monoclonal antibodies and by T1 oligon...

Full description

Saved in:
Bibliographic Details
Published in:Journal of general virology 1982-08, Vol.61 (2), p.167-176
Main Authors: Minor, P. D, Schild, G. C, Ferguson, Morag, MacKay, Alison, Magrath, D. I, John, Ann, Yates, P. J, Spitz, M
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal
Antigens, Viral - immunology
Epitopes
Immunodiffusion
Neutralization Tests
Oligoribonucleotides - analysis
Poliovirus - genetics
Poliovirus - immunology
Ribonuclease T1
RNA, Viral - analysis
Species Specificity
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:National Institute for Biological Standards and Control, Holly Hill, Hampstead, London NW3 6RB, U.K. Considerable genetic and antigenic heterogeneity was detected among a collection of 17 poliovirus type 3 strains isolated between 1939 and 1958 in studies using monoclonal antibodies and by T1 oligonucleotide mapping. Heterogeneity was detected even amongst a collection of nine viruses designated Saukett and assumed to originate from the same prototype virus. The monoclonal antibodies were found to differ in their strain specificities for poliovirus type 3 strains in virus neutralization or single-radial-immunodiffusion tests. Relationships between strains detected in this way were in general consistent with those detected by oligonucleotide mapping. One of the monoclonal antibodies (NIBp 56) was able to distinguish between certain Saukett virus strains which differed by as little as a single specific oligonucleotide. The heterogeneity detected amongst Saukett viruses is of potential practical importance since these strains are used widely in the manufacture of inactivated poliovirus vaccine. Keywords: Saukett type 3 poliovirus, T1 oligonucleotides, monoclonal antibodies, neutralization Received 27 November 1981; accepted 8 March 1982.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-61-2-167