Cdc42 and mDia3 regulate microtubule attachment to kinetochores

During mitosis, the mitotic spindle, a bipolar structure composed of microtubules (MTs) and associated motor proteins, segregates sister chromatids to daughter cells. Initially some MTs emanating from one centrosome attach to the kinetochore at the centromere of one of the duplicated chromosomes. Th...

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Bibliographic Details
Published in:Nature 2004-04, Vol.428 (6984), p.767-771
Main Authors: Narumiya, Shuh, Yasuda, Shingo, Oceguera-Yanez, Fabian, Kato, Takayuki, Okamoto, Muneo, Yonemura, Shigenobu, Terada, Yasuhiko, Ishizaki, Toshimasa
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Carrier Proteins - genetics
Carrier Proteins - metabolism
cdc42 GTP-Binding Protein - metabolism
Cell cycle, cell proliferation
Cell division
Cell physiology
Cellular biology
Chromosomes
Cytoskeleton
Fundamental and applied biological sciences. Psychology
Guanosine 5'-O-(3-Thiotriphosphate) - metabolism
Guanosine Diphosphate - metabolism
HeLa Cells
Humanities and Social Sciences
Humans
Kinetochores - metabolism
letter
Mice
Microtubules - metabolism
Mitosis - drug effects
Molecular and cellular biology
multidisciplinary
Mutation
NIH 3T3 Cells
Proteins
rho GTP-Binding Proteins - genetics
rho GTP-Binding Proteins - metabolism
Science
Science (multidisciplinary)
Signal Transduction - drug effects
Spindle Apparatus - metabolism
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Summary:During mitosis, the mitotic spindle, a bipolar structure composed of microtubules (MTs) and associated motor proteins, segregates sister chromatids to daughter cells. Initially some MTs emanating from one centrosome attach to the kinetochore at the centromere of one of the duplicated chromosomes. This attachment allows rapid poleward movement of the bound chromosome. Subsequent attachment of the sister kinetochore to MTs growing from the other centrosome results in the bi-orientation of the chromosome, in which interactions between kinetochores and the plus ends of MTs are formed and stabilized. These processes ensure alignment of chromosomes during metaphase and their correct segregation during anaphase. Although many proteins constituting the kinetochore have been identified and extensively studied, the signalling responsible for MT capture and stabilization is unclear. Small GTPases of the Rho family regulate cell morphogenesis by organizing the actin cytoskeleton and regulating MT alignment and stabilization. We now show that one member of this family, Cdc42, and its effector, mDia3, regulate MT attachment to kinetochores.
ISSN:0028-0836
1476-4687
DOI:10.1038/nature02452