Changes in Crested Wheatgrass Root Exudation Caused by Flood, Drought, and Nutrient Stress

• Published in: Journal of environmental quality 2007-05, Vol.36 (3), p.904-912
• Main Authors: Henry, A, Doucette, W, Norton, J, Bugbee, B
• Format: Article
• Language: English
• Subjects: Acids - chemistry; Acids - metabolism; Agropyron cristatum; Amino acids; ammonia; Biodegradation, Environmental; Carbon - metabolism; Cells; chemical composition; Drought; flooded conditions; Floods; Gas chromatography; Grasses; Hypoxia; leachates; Mass spectrometry; nitrates; Nutrients; Organic acids; Organic carbon; Organic contaminants; Oxalic acid; Phytoremediation; plant growth; plant health; Plant Roots - chemistry; Plant Roots - metabolism; Plant Shoots - chemistry; Plant Shoots - metabolism; Poaceae - growth & development; Poaceae - metabolism; polluted soils; potassium; Rhizosphere; root exudates; Soil contaminants; Soil contamination; soil pollution; Water - metabolism; Water stress
• ISSN: 0047-2425; 1537-2537
• DOI: 10.2134/jeq2006.0425sc

Root exudates can chelate inorganic soil contaminants, change rhizosphere pH, and may increase degradation of organic contaminants by microbial cometabolism. Root-zone stress may increase exudation and enhance phytoremediation. We studied the effects of low K+, high NH4+/NO3- ratio, drought, and flooding on the quantity and composition of exudates. Crested wheatgrass (Agropyron cristatum) was grown in Ottawa sand in sealed, flow-through glass columns under axenic conditions for 70 d. Root exudates were collected and analyzed for total organic carbon (TOC) and organic acid content to compare treatment effects. Plants in the low K+ treatment exuded 60% more TOC per plant per day (p = 0.01) than the unstressed control. Drought stress increased cumulative TOC exuded per gram dry plant by 71% (p = 0.05). The flooded treatment increased TOC exuded per gram dry plant by 45%, although this was not statistically significant based on the two replicate plants in this treatment. Exudation from the high NH4+/NO3- ratio treatment was 10% less than the control. Exudation rates in this study ranged from 8 to 50% of rates in four other published studies. Gas chromatography-mass spectrometry (GC-MS) analysis indicated that malic acid was the predominant organic acid exuded. Fumaric, malonic, succinic, and oxalic acids were also detected in the exudates of all treatments. These results demonstrate that nutrient and water stress have significant effects on the quantity and composition of root exudates. Cultural manipulations to induce stress may change the quantity of root exudates and thus increase the effectiveness of phytoremediation.