Cellulase elicitor induced accumulation of capsidiol in Capsicum annumm L. suspension cultures

• Published in: Biotechnology letters 2008-05, Vol.30 (5), p.961-965
• Main Author: Ma, Choong Je
• Format: Article
• Language: English
• Subjects: Acetates - metabolism; Applied Microbiology; Aristolochic Acids - metabolism; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Capsicum; Capsicum - metabolism; Capsicum annuum; Cell culture; Cells, Cultured; Cellulase - metabolism; Chromatography, Gas; Culture media; Cyclopentanes - metabolism; Enzymes; Fundamental and applied biological sciences. Psychology; Life Sciences; Microbiology; Original Research Paper; Oxylipins - metabolism; Peptides - metabolism; Phospholipase A2 Inhibitors; Phospholipases A2 - metabolism; Plant biology; Plant resistance; Sesquiterpenes - isolation & purification; Sesquiterpenes - metabolism; Time Factors; Wasp Venoms - metabolism
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-007-9624-y

When growth-phase cell suspension cultures of Capsicum annuum were treated with cellulase-elicitor preparation at 3 μg/ml, the level of capsidiol was transiently increased in the culture media rather than in the cells reaching its maximum approx 24 h after treatment. With methyl jasmonate it took 18 h. Elicitor treatment doubled phospholiphase A₂ (PLA₂) activity but simultaneous treatment with aristolochic acid, a PLA₂ inhibitor, inhibited sesquiterpenoid accumulation as well as PLA₂ activity. Mastoparan, a G protein activator, treatment also increased PLA₂ activity and capsidiol production. Taken together, the present study shows that induction of capsidiol production in the C. annuum is mediated by PLA₂ activation.