Combinatorial Synthesis of Peptide Arrays onto a Microchip

Arrays promise to advance biology through parallel screening for binding partners. We show the combinatorial in situ synthesis of 40,000 peptide spots per square centimeter on a microchip. Our variant Merrifield synthesis immobilizes activated amino acids as monomers within particles, which are succ...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2007-12, Vol.318 (5858), p.1888-1888
Main Authors: Beyer, Mario, Nesterov, Alexander, Block, Ines, König, Kai, Felgenhauer, Thomas, Fernandez, Simon, Leibe, Klaus, Torralba, Gloria, Hausmann, Michael, Trunk, Ulrich, Lindenstruth, Volker, Bischoff, F. Ralf, Stadler, Volker, Breitling, Frank
Format: Article
Language:English
Subjects:
Amino acids
Amino Acids - chemistry
Antibodies
Biochemistry
Brevia
Combinatorial Chemistry Techniques
Electric fields
Electricity
Electrodes
Esters
Integrated circuits
Miniaturization
Molecular biology
Monomers
Particle density
Particle diffusion
Peptides
Peptides - chemical synthesis
Pixels
Protein Array Analysis - methods
Proteomics
Research methodology
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Summary:Arrays promise to advance biology through parallel screening for binding partners. We show the combinatorial in situ synthesis of 40,000 peptide spots per square centimeter on a microchip. Our variant Merrifield synthesis immobilizes activated amino acids as monomers within particles, which are successively attracted by electric fields generated on each pixel electrode of the chip. With all different amino acids addressed, particles are melted at once to initiate coupling. Repetitive coupling cycles should allow for the translation of whole proteomes into arrays of overlapping peptides that could be used for proteome research and antibody profiling.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1149751