Cloning and sequence analysis of the gene encoding (6-4)photolyase from Dunaliella salina

DNA photolyase can repair UV-induced DNA damage in a light-dependent manner. A cDNA of (6-4)photolyase from Dunaliella salina (GenBank accession number: AY845324) was cloned, sequenced and its amino acid sequence was deduced. The derived amino acid sequence showed high homology with other (6-4)photo...

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Bibliographic Details
Published in:Biotechnology letters 2006-03, Vol.28 (5), p.309-314
Main Authors: Yi, Yi, Yi, Cao, Qian, Li, Min, Liu, Long, Cheng, Linhan, Bai, Zhirong, Yang, Dairong, Qiao
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Base Sequence
Biological and medical sciences
Biotechnology
Chlorophyta - enzymology
Cloning
Cloning, Molecular - methods
Computer Simulation
Conserved Sequence
Deoxyribodipyrimidine Photo-Lyase - chemistry
Deoxyribodipyrimidine Photo-Lyase - genetics
Deoxyribonucleic acid
DNA
DNA repair
Dunaliella salina
Fundamental and applied biological sciences. Psychology
Genes
Models, Chemical
Models, Molecular
Molecular Sequence Data
Sequence Analysis, Protein
Sequence Homology, Amino Acid
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Summary:DNA photolyase can repair UV-induced DNA damage in a light-dependent manner. A cDNA of (6-4)photolyase from Dunaliella salina (GenBank accession number: AY845324) was cloned, sequenced and its amino acid sequence was deduced. The derived amino acid sequence showed high homology with other (6-4)photolyases and a predicted 3D model was constructed by homology modeling.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-005-5716-8