Cultivation of microorganisms in the cultural medium made from squid internal organs and accumulation of polyunsaturated fatty acids in the cells

• Published in: Biotechnology letters 2005-07, Vol.27 (13), p.933-941
• Main Authors: MORITA, Naoki, ICHISE, Nobutoshi, YUMOTO, Isao, YANO, Yutaka, OHGIYA, Satoru, OKUYAMA, Hidetoshi
• Format: Article
• Language: English
• Subjects: Animals; Bacteria; Bacteria - drug effects; Bacteria - growth & development; Bacteria - metabolism; Biological and medical sciences; Biosynthesis; Biotechnology; Culture media; Culture Media - chemistry; Culture Media - pharmacology; Decapodiformes - chemistry; Docosahexaenoic Acids - metabolism; E coli; Eicosapentaenoic Acid - metabolism; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - growth & development; Escherichia coli - metabolism; Fatty acids; Fatty Acids, Unsaturated - chemistry; Fatty Acids, Unsaturated - metabolism; Fatty Acids, Unsaturated - pharmacology; Fundamental and applied biological sciences. Psychology; Lipid Metabolism; Lipids; Lipids - chemistry; Microbiology; Microorganisms; Moritella - drug effects; Moritella - growth & development; Moritella - metabolism; Polyunsaturated fatty acids; Shewanella putrefaciens - drug effects; Shewanella putrefaciens - growth & development; Shewanella putrefaciens - metabolism; Species Specificity; Vibrio - drug effects; Vibrio - growth & development; Vibrio - metabolism
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-005-7187-3

The disposal and more efficient utilization of marine wastes is becoming increasingly serious. A culture media for microorganisms has been prepared from squid internal organs that are rich in polyunsaturated fatty acids (PUFAs). Both freshwater and marine bacteria grew well in this medium and some bacteria accumulated PUFAs in their lipids, suggesting uptake of exogenous PUFAs. Higher PUFA accumulations were observed in Escherichia coli mutant cells defective either in unsaturated fatty acid biosynthesis or fatty acid degradation, or both, compared to those without these mutations. Therefore, PUFA accumulation in cells can be improved by genetic modification of fatty acid metabolism in the bacteria. Squid internal organs would be a good source of medium, not only for marine bacteria but also for freshwater bacteria, and that this process may be advantageous to make efficient use of the fishery wastes and to produce PUFA-containing microbial cells and lipids.