Comparison of hexamethyldisilazane and critical point drying treatments for SEM analysis of anaerobic biofilms and granular sludge

We present a fast procedure for scanning electron microscopy (SEM) analysis in which hexamethyldisilazane (HMDS) solvent, instead of the critical point drying, is used to remove liquids from a microbiological specimen. The results indicate that the HMDS solvent is suitable for drying samples of anae...

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Bibliographic Details
Published in:Journal of electron microscopy 2003-09, Vol.52 (4), p.429-433
Main Authors: Araujo, Juliana C., Téran, Francisco C., Oliveira, Roberto A., Nour, Edson A. A., Montenegro, Martha A. P., Campos, José R., Vazoller, Rosana F.
Format: Article
Language:English
Subjects:
anaerobic biofilms
Bacteria, Anaerobic - ultrastructure
Biofilms - growth & development
Bioreactors
critical point drying method
Desiccation
granular sludge
HMDS treatment
Methanosarcinales - ultrastructure
Microscopy, Electron, Scanning - methods
Organosilicon Compounds
SEM analysis
Sewage - microbiology
Tissue Fixation
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Description
Summary:We present a fast procedure for scanning electron microscopy (SEM) analysis in which hexamethyldisilazane (HMDS) solvent, instead of the critical point drying, is used to remove liquids from a microbiological specimen. The results indicate that the HMDS solvent is suitable for drying samples of anaerobic cells for examination by SEM and does not cause cell structure disruption.
ISSN:0022-0744
1477-9986
2050-5701
DOI:10.1093/jmicro/52.4.429