Adult pancreatic β-cells are formed by self-duplication rather than stem-cell differentiation

How tissues generate and maintain the correct number of cells is a fundamental problem in biology. In principle, tissue turnover can occur by the differentiation of stem cells, as is well documented for blood, skin and intestine, or by the duplication of existing differentiated cells. Recent work on...

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Published in:Nature 2004-05, Vol.429 (6987), p.41-46
Main Authors: Melton, Douglas A, Dor, Yuval, Brown, Juliana, Martinez, Olga I
Format: Article
Language:English
Subjects:
Aging - physiology
Animals
Biological and medical sciences
Cell Count
Cell Differentiation
Cell differentiation, maturation, development, hematopoiesis
Cell Division
Cell Lineage - physiology
Cell physiology
Cellular biology
Fundamental and applied biological sciences. Psychology
Humanities and Social Sciences
Islets of Langerhans - cytology
Islets of Langerhans - metabolism
Mice
Mice, Transgenic
Molecular and cellular biology
multidisciplinary
Pancreas
Pancreatectomy
Pluripotent Stem Cells - cytology
Recombination, Genetic
Regeneration - physiology
Science
Science (multidisciplinary)
Stem cells
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description How tissues generate and maintain the correct number of cells is a fundamental problem in biology. In principle, tissue turnover can occur by the differentiation of stem cells, as is well documented for blood, skin and intestine, or by the duplication of existing differentiated cells. Recent work on adult stem cells has highlighted their potential contribution to organ maintenance and repair. However, the extent to which stem cells actually participate in these processes in vivo is not clear. Here we introduce a method for genetic lineage tracing to determine the contribution of stem cells to a tissue of interest. We focus on pancreatic β-cells, whose postnatal origins remain controversial. Our analysis shows that pre-existing β-cells, rather than pluripotent stem cells, are the major source of new β-cells during adult life and after pancreatectomy in mice. These results suggest that terminally differentiated β-cells retain a significant proliferative capacity in vivo and cast doubt on the idea that adult stem cells have a significant role in β-cell replenishment.
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subjects Aging - physiology
Animals
Biological and medical sciences
Cell Count
Cell Differentiation
Cell differentiation, maturation, development, hematopoiesis
Cell Division
Cell Lineage - physiology
Cell physiology
Cellular biology
Fundamental and applied biological sciences. Psychology
Humanities and Social Sciences
Islets of Langerhans - cytology
Islets of Langerhans - metabolism
Mice
Mice, Transgenic
Molecular and cellular biology
multidisciplinary
Pancreas
Pancreatectomy
Pluripotent Stem Cells - cytology
Recombination, Genetic
Regeneration - physiology
Science
Science (multidisciplinary)
Stem cells
title Adult pancreatic β-cells are formed by self-duplication rather than stem-cell differentiation
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