A DNA microarray for differentiation of the Chinese medicinal herb Dendrobium officinale (Fengdou Shihu) by its 5 S ribosomal DNA intergenic spacer region

A DNA microarray was constructed for high‐throughput identification of the plant resource of commercial FDSH [Fengdu Shihu (Dendrobium officinale)]. The 5 S rDNA (ribosomal DNA) intergenic spacer region in D. officinale, D. nobile, D. moniliforme, D. hercoglossum, D. williamsonii, D. capillipes, D....

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Published in:Biotechnology and applied biochemistry 2008-02, Vol.49 (2), p.149-154
Main Authors: Sze, Stephen Cho-Wing, Zhang, Kalin Yan-Bo, Shaw, Pang-Chui, But, Paul Pui-Hay, Ng, Tzi-Bun, Tong, Yao
Format: Article
Language:English
Subjects:
5 S ribosomal DNA gene
authentification by DNA microarray
authentification by DNA microarray
Chinese medicine
Biological and medical sciences
Biotechnology
Chinese medicine
Dendrobium officinale (Fengdu Shihu
FDSH
Fundamental and applied biological sciences. Psychology
identification of plant adulterants
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Summary:A DNA microarray was constructed for high‐throughput identification of the plant resource of commercial FDSH [Fengdu Shihu (Dendrobium officinale)]. The 5 S rDNA (ribosomal DNA) intergenic spacer region in D. officinale, D. nobile, D. moniliforme, D. hercoglossum, D. williamsonii, D. capillipes, D. wilsonii and D. jenkinsii was amplified by a single primer pair and sequenced. The sequences showed polymorphism. They were incorporated on a glass slide and hybridized with fluorescently labelled 5 S sequences from commercial Shihu. The DNA microarray enabled the differentiation of D. officinale from the other species tested. FDSH could thus be distinguished from its adulterants. It is evident that DNA microarrays provide a high‐throughput and reliable approach for the identification of plant resources, and the method presented here is useful for the authentication of FDSH.
ISSN:0885-4513
1470-8744
DOI:10.1042/BA20070059