Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper: Excel-based tool using pair-wise correlations

The stability of standard gene expression is an elementary prerequisite for internal standardisation of target gene expression data and many so called housekeeping genes with assumed stable expression can exhibit either up- or down-regulation under some experimental conditions. The developed, and he...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology letters 2004-03, Vol.26 (6), p.509-515
Main Authors: PFAFFL, Michael W, TICHOPAD, Ales, PRGOMET, Christian, NEUVIANS, Tanja P
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biotechnology
Cattle
Corpus Luteum - physiology
Data processing
Female
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression - physiology
Gene Expression Profiling - standards
Genes - physiology
Reverse Transcriptase Polymerase Chain Reaction - standards
RNA - genetics
Software
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The stability of standard gene expression is an elementary prerequisite for internal standardisation of target gene expression data and many so called housekeeping genes with assumed stable expression can exhibit either up- or down-regulation under some experimental conditions. The developed, and herein presented, software called BestKeeper determines the best suited standards, out of ten candidates, and combines them into an index. The index can be compared with further ten target genes to decide, whether they are differentially expressed under an applied treatment. All data processing is based on crossing points. The BestKeeper software tool was validated on four housekeeping genes and 10 members of the somatotropic axis differentially expressed in bovine corpora lutea total RNA. The BestKeeper application and necessary information about data processing and handling can be downloaded on http://www.wzw.tum.de/gene-quantification/bestkeeper.html.
ISSN:0141-5492
1573-6776
DOI:10.1023/B:BILE.0000019559.84305.47