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Fusion of bone-marrow-derived cells with Purkinje neurons, cardiomyocytes and hepatocytes
Recent studies have suggested that bone marrow cells possess a broad differentiation potential, being able to form new liver cells, cardiomyocytes and neurons. Several groups have attributed this apparent plasticity to 'transdifferentiation'. Others, however, have suggested that cell fusio...
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| Published in: | Nature 2003-10, Vol.425 (6961), p.968-973 |
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| Main Authors: | , , , , , , , , |
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| container_end_page | 973 |
| container_issue | 6961 |
| container_start_page | 968 |
| container_title | Nature |
| container_volume | 425 |
| creator | Alvarez-Buylla, Arturo Alvarez-Dolado, Manuel Pardal, Ricardo Garcia-Verdugo, Jose M Fike, John R Lee, Hyun O Pfeffer, Klaus Lois, Carlos Morrison, Sean J |
| description | Recent studies have suggested that bone marrow cells possess a broad differentiation potential, being able to form new liver cells, cardiomyocytes and neurons. Several groups have attributed this apparent plasticity to 'transdifferentiation'. Others, however, have suggested that cell fusion could explain these results. Using a simple method based on Cre/lox recombination to detect cell fusion events, we demonstrate that bone-marrow-derived cells (BMDCs) fuse spontaneously with neural progenitors in vitro. Furthermore, bone marrow transplantation demonstrates that BMDCs fuse in vivo with hepatocytes in liver, Purkinje neurons in the brain and cardiac muscle in the heart, resulting in the formation of multinucleated cells. No evidence of transdifferentiation without fusion was observed in these tissues. These observations provide the first in vivo evidence for cell fusion of BMDCs with neurons and cardiomyocytes, raising the possibility that cell fusion may contribute to the development or maintenance of these key cell types. |
| doi_str_mv | 10.1038/nature02069 |
| format | article |
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Several groups have attributed this apparent plasticity to 'transdifferentiation'. Others, however, have suggested that cell fusion could explain these results. Using a simple method based on Cre/lox recombination to detect cell fusion events, we demonstrate that bone-marrow-derived cells (BMDCs) fuse spontaneously with neural progenitors in vitro. Furthermore, bone marrow transplantation demonstrates that BMDCs fuse in vivo with hepatocytes in liver, Purkinje neurons in the brain and cardiac muscle in the heart, resulting in the formation of multinucleated cells. No evidence of transdifferentiation without fusion was observed in these tissues. These observations provide the first in vivo evidence for cell fusion of BMDCs with neurons and cardiomyocytes, raising the possibility that cell fusion may contribute to the development or maintenance of these key cell types.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/nature02069</identifier><identifier>PMID: 14555960</identifier><identifier>CODEN: NATUAS</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Animal cells ; Animals ; Biological and medical sciences ; Bone marrow ; Bone Marrow Cells - cytology ; Bone Marrow Transplantation ; Cell cultures. Hybridization. Fusion ; Cell Differentiation ; Cell Fusion ; Cells ; Fundamental and applied biological sciences. Psychology ; Giant Cells - cytology ; Hepatocytes - cytology ; Humanities and Social Sciences ; letter ; Mice ; Mice, Inbred C57BL ; Models, Biological ; Molecular and cellular biology ; multidisciplinary ; Myocytes, Cardiac - cytology ; Neurons ; Purkinje Cells - cytology ; Science ; Science (multidisciplinary) ; Stem Cells - cytology</subject><ispartof>Nature, 2003-10, Vol.425 (6961), p.968-973</ispartof><rights>Macmillan Magazines Ltd. 2003</rights><rights>2003 INIST-CNRS</rights><rights>COPYRIGHT 2003 Nature Publishing Group</rights><rights>Copyright Macmillan Journals Ltd. 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Several groups have attributed this apparent plasticity to 'transdifferentiation'. Others, however, have suggested that cell fusion could explain these results. Using a simple method based on Cre/lox recombination to detect cell fusion events, we demonstrate that bone-marrow-derived cells (BMDCs) fuse spontaneously with neural progenitors in vitro. Furthermore, bone marrow transplantation demonstrates that BMDCs fuse in vivo with hepatocytes in liver, Purkinje neurons in the brain and cardiac muscle in the heart, resulting in the formation of multinucleated cells. No evidence of transdifferentiation without fusion was observed in these tissues. These observations provide the first in vivo evidence for cell fusion of BMDCs with neurons and cardiomyocytes, raising the possibility that cell fusion may contribute to the development or maintenance of these key cell types.</description><subject>Animal cells</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bone marrow</subject><subject>Bone Marrow Cells - cytology</subject><subject>Bone Marrow Transplantation</subject><subject>Cell cultures. Hybridization. Fusion</subject><subject>Cell Differentiation</subject><subject>Cell Fusion</subject><subject>Cells</subject><subject>Fundamental and applied biological sciences. 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| identifier | ISSN: 0028-0836 |
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| language | eng |
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| source | Nature Journals Online |
| subjects | Animal cells Animals Biological and medical sciences Bone marrow Bone Marrow Cells - cytology Bone Marrow Transplantation Cell cultures. Hybridization. Fusion Cell Differentiation Cell Fusion Cells Fundamental and applied biological sciences. Psychology Giant Cells - cytology Hepatocytes - cytology Humanities and Social Sciences letter Mice Mice, Inbred C57BL Models, Biological Molecular and cellular biology multidisciplinary Myocytes, Cardiac - cytology Neurons Purkinje Cells - cytology Science Science (multidisciplinary) Stem Cells - cytology |
| title | Fusion of bone-marrow-derived cells with Purkinje neurons, cardiomyocytes and hepatocytes |
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