Fusion of Cells by Flipped SNAREs

The SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) hypothesis suggests that pairs of proteins known as vesicle (v-) SNAREs and target membrane (t-) SNAREs interact specifically to control and mediate intracellular membrane fusion events. Here, cells expressing the inte...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2003-06, Vol.300 (5626), p.1745-1749
Main Authors: Hu, Chuan, Ahmed, Mahiuddin, Melia, Thomas J., Söllner, Thomas H., Mayer, Thomas, Rothman, James E.
Format: Article
Language:English
Subjects:
Animals
Antigens, Surface - chemistry
Antigens, Surface - metabolism
Biological and medical sciences
Cell Fusion
Cell Membrane - metabolism
Cell membranes
Cell nucleus
Cells
Cercopithecus aethiops
Coding
COS Cells
Cytoplasm
Endoplasmic Reticulum - metabolism
Fundamental and applied biological sciences. Psychology
Genes
Glycosylation
HIV 1
Membrane Fusion - physiology
Membrane proteins
Membrane Proteins - chemistry
Membrane Proteins - genetics
Membrane Proteins - metabolism
Membranes
Molecular and cellular biology
Mutation
Nerve Tissue Proteins - chemistry
Nerve Tissue Proteins - genetics
Nerve Tissue Proteins - metabolism
Neurotransmitters
Protein Binding
Protein Folding
Protein Structure, Tertiary
Proteins
Quality Control
R-SNARE Proteins
Recombinant Fusion Proteins - metabolism
SNARE proteins
Synaptosomal-Associated Protein 25
Syntaxin 1
T lymphocytes
Transfection
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Description
Summary:The SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) hypothesis suggests that pairs of proteins known as vesicle (v-) SNAREs and target membrane (t-) SNAREs interact specifically to control and mediate intracellular membrane fusion events. Here, cells expressing the interacting domains of v- and t-SNAREs on the cell surface were found to fuse spontaneously, demonstrating that SNAREs are sufficient to fuse biological membranes.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1084909