Amphiphysin 2 (Bin1) and T-Tubule Biogenesis in Muscle

• Published in: Science (American Association for the Advancement of Science) 2002-08, Vol.297 (5584), p.1193-1196
• Main Authors: Lee, Eunkyung, Marcucci, Melissa, Daniell, Laurie, Pypaert, Marc, Weisz, Ora A., Ochoa, Gian-Carlo, Farsad, Khashayar, Wenk, Markus R., De Camilli, Pietro
• Format: Article
• Language: English
• Subjects: Animals; B lymphocytes; Biological and medical sciences; Biology; Caveolin 3; Caveolins; Caveolins - metabolism; Cell Differentiation; Cell Line; Cell Membrane - metabolism; Cell Membrane Structures - metabolism; Cell Membrane Structures - ultrastructure; Cell membranes; Cellular differentiation; Cellular immunity; CHO Cells; Cricetinae; Developmental cytology; Dynamins; Exons; Formation; Fundamental and applied biological sciences. Psychology; GTP Phosphohydrolases - metabolism; Individualized Instruction; Liposomes; Liposomes - metabolism; Membranes; Mice; Microscopy, Electron; Morphogenesis; Muscle Development; Muscle fibers; Muscle, Skeletal - metabolism; Muscle, Skeletal - ultrastructure; Muscles; Muscular system; Nerve Tissue Proteins - chemistry; Nerve Tissue Proteins - genetics; Nerve Tissue Proteins - metabolism; Phosphatidylinositol 4,5-Diphosphate - metabolism; Physiological aspects; Protein Isoforms; Protein Structure, Tertiary; Recombinant Fusion Proteins - metabolism; RNA, Small Interfering; RNA, Untranslated - metabolism; Striated muscle. Tendons; Transfection; Vertebrates: osteoarticular system, musculoskeletal system
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1071362

In striated muscle, the plasma membrane forms tubular invaginations (transverse tubules or T-tubules) that function in depolarization-contraction coupling. Caveolin-3 and amphiphysin were implicated in their biogenesis. Amphiphysin isoforms have a putative role in membrane deformation at endocytic sites. An isoform of amphiphysin 2 concentrated at T-tubules induced tubular plasma membrane invaginations when expressed in non-muscle cells. This property required exon 10, a phosphoinositide-binding module. In developing myotubes, amphiphysin 2 and caveolin-3 segregated in tubular and vesicular portions of the T-tubule system, respectively. These findings support a role of the bilayer-deforming properties of amphiphysin at T-tubules and, more generally, a physiological role of amphiphysin in membrane deformation.