Elemental and Redox Analysis of Single Bacterial Cells by X-ray Microbeam Analysis

High-energy x-ray fluorescence measurements were used to make elemental maps and qualitative chemical analyses of individual Pseudomonas fluorescens strain NCIMB 11764 cells. Marked differences between planktonic and adhered cells were seen in the morphology, elemental composition, and sensitivity t...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2004-10, Vol.306 (5696), p.686-687
Main Authors: Kemner, Kenneth M., Kelly, Shelly D., Lai, Barry, Maser, Joerg, O'Loughlin, Edward J., Sholto-Douglas, Deirdre, Cai, Zhonghou, Schneegurt, Mark A., Kulpa, Charles F., Nealson, Kenneth H.
Format: Article
Language:English
Subjects:
Bacteria
Bacterial Adhesion
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biochemistry
Biofilms
Biological and medical sciences
Calcium - analysis
Cellular biology
Cellular metabolism
Chemical composition
Chromium - analysis
Chromium - pharmacology
Elements
Energy
Fluorescence
Fundamental and applied biological sciences. Psychology
Materials
Microbiology
Microorganisms
Microscopy
Organic Chemistry
Oxidation-Reduction
Phosphorus - analysis
Physiological aspects
Potassium
Prokaryotic cells
Pseudomonas fluorescens
Pseudomonas fluorescens - chemistry
Pseudomonas fluorescens - cytology
Pseudomonas fluorescens - physiology
Spatial resolution
Spectrometry, X-Ray Emission
Spectrum analysis
Technology application
X-ray spectroscopy
X-rays
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Summary:High-energy x-ray fluorescence measurements were used to make elemental maps and qualitative chemical analyses of individual Pseudomonas fluorescens strain NCIMB 11764 cells. Marked differences between planktonic and adhered cells were seen in the morphology, elemental composition, and sensitivity to Cr(VI) of hydrated cells at spatial scales of 150 nm. This technology can be applied to natural geomicrobiological systems.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1103524