Modulation of Phospholipid Signaling by GLABRA2 in Root-Hair Pattern Formation

• Published in: Science (American Association for the Advancement of Science) 2003-05, Vol.300 (5624), p.1427-1430
• Main Authors: Ohashi, Yohei, Oka, Atsuhiro, Rodrigues-Pousada, Renato, Possenti, Marco, Ruberti, Ida, Morelli, Giorgio, Aoyama, Takashi
• Format: Article
• Language: English
• Subjects: Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis - metabolism; Arabidopsis Proteins - chemistry; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Biological and medical sciences; Dexamethasone - pharmacology; DNA; Epidermal cells; Epidermis; Feedback (Response); Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Plant; Genes; Genes, Plant; Hair; Hair cells; Homeodomain Proteins - chemistry; Homeodomain Proteins - genetics; Homeodomain Proteins - metabolism; Lipids; Morphogenesis; Observations; Phosphates; Phospholipase D - genetics; Phospholipase D - metabolism; Phospholipids - metabolism; Plant cell development; Plant cells; Plant Epidermis - cytology; Plant Epidermis - metabolism; Plant growth. Development of the storage organs; Plant physiology and development; Plant roots; Plant Roots - cytology; Plant Roots - growth & development; Plant Roots - metabolism; Plants; Plants, Genetically Modified; Promoter Regions, Genetic; Root hairs; Signal Transduction; Transgenic plants; Vegetative apparatus, growth and morphogenesis. Senescence
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1083695

The root-hair pattern of Arabidopsis is determined through a regulatory circuit composed of transcription factor genes. The homeobox gene GLABRA2 (GL2) has been considered a key component, acting farthest downstream in this regulation. GL2 modified to include a transactivating function caused epidermal cells to develop ectopic root hairs or root hair-like structures. With this system, the phospholipase Dζ1 gene (AtPLDζ1) was identified as a direct target of GL2. Inducible expression of AtPLDζ1 promoted ectopic root-hair initiation. We conclude that GL2 exerts its regulatory effect on root-hair development through modulation of phospholipid signaling.