DETERMINATION OF THE ID50 VALUES OF ANTIBACTERIAL AGENTS IN AGAR

Microorganisms were plated on agar plates containing various concentrations of an antimicrobial drug and inhibition of growth was determined at each drug concentration. The ID50 value and the gradient of the line were calculated by the least square method. When 200-800 bacterial cells were inoculate...

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Bibliographic Details
Published in:Journal of antibiotics 1978, Vol.31(12), pp.1299-1303
Main Authors: KATO, TAKAKO, KURASHIGE, SATONORI, CHABBERT, Y. A., MITSUHASHI, SUSUMU
Format: Article
Language:English
Subjects:
Agar
Anti-Bacterial Agents - pharmacology
Bacteria - drug effects
Bacteria - growth & development
Culture Media
Escherichia coli - drug effects
Escherichia coli - growth & development
Microbial Sensitivity Tests - methods
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Summary:Microorganisms were plated on agar plates containing various concentrations of an antimicrobial drug and inhibition of growth was determined at each drug concentration. The ID50 value and the gradient of the line were calculated by the least square method. When 200-800 bacterial cells were inoculated on an agar plate, growth inhibition corresponded linearly with the log concentration of a drug within the range of 5-95% inhibition. The ID50 value and the gradient obtained were reproducible and reliable using microorganisms at stationary phase of growth with all tested bacterial species and all tested antimicrobial agents. It was found that the ID50 values of drugs were more reproducible and may be more reliable than the MIC (minimum inhibitory concentration) values of the drugs.
ISSN:0021-8820
1881-1469
DOI:10.7164/antibiotics.31.1299