Maize Genome Sequencing by Methylation Filtration

Gene enrichment strategies offer an alternative to sequencing large and repetitive genomes such as that of maize. We report the generation and analysis of nearly 100,000 undermethylated (or methylation filtration) maize sequences. Comparison with the rice genome reveals that methylation filtration r...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2003-12, Vol.302 (5653), p.2115-2117
Main Authors: Palmer, Lance E., Rabinowicz, Pablo D., O'Shaughnessy, Andrew L., Balija, Vivekanand S., Nascimento, Lidia U., Dike, Sujit, de la Bastide, Melissa, Martienssen, Robert A., McCombie, W. Richard
Format: Article
Language:English
Subjects:
Algorithms
Base sequence
Biological and medical sciences
Chromosomes, Artificial, Bacterial
Classical genetics, quantitative genetics, hybrids
Cloning, Molecular
Complementary DNA
Computational Biology
Conserved Sequence
Contig Mapping
Corn
CpG Islands
Crops
Databases, Nucleic Acid
Disproportionate Representation
DNA Methylation
DNA Transposable Elements
DNA, Chloroplast - genetics
DNA, Complementary
DNA, Mitochondrial - genetics
DNA, Plant - genetics
Escherichia coli - genetics
Exons
Expressed Sequence Tags
Filters
Fundamental and applied biological sciences. Psychology
Genes
Genes, Plant
Genetic aspects
Genetics of eukaryotes. Biological and molecular evolution
Genome, Plant
Genomes
Genomic Library
Genomics
Libraries
Methylation
Nucleotide sequence
Oryza - genetics
Polymerase chain reaction
Pteridophyta, spermatophyta
Repetitive Sequences, Nucleic Acid
Retroelements
Reverse Transcriptase Polymerase Chain Reaction
Rice
Sequence Analysis, DNA - methods
Sequencing
Transposons
Vegetals
Zea mays
Zea mays - genetics
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Description
Summary:Gene enrichment strategies offer an alternative to sequencing large and repetitive genomes such as that of maize. We report the generation and analysis of nearly 100,000 undermethylated (or methylation filtration) maize sequences. Comparison with the rice genome reveals that methylation filtration results in a more comprehensive representation of maize genes than those that result from expressed sequence tags or transposon insertion sites sequences. About 7% of the repetitive DNA is unmethylated and thus selected in our libraries, but potentially active transposons and unmethylated organelle genomes can be identified. Reverse transcription polymerase chain reaction can be used to finish the maize transcriptome.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1091265