Indole-3-acetic acid biosynthesis in the mutant maize orange pericarp, a tryptophan auxotroph

The maize mutant orange pericarp is a tryptophan auxotroph, which results from mutation of two unlinked loci of tryptophan synthase B. This mutant was used to test the hypothesis that tryptophan is the precursor to the plant hormone indole-3-acetic acid (IAA). Total IAA in aseptically grown mutant s...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1991-11, Vol.254 (5034), p.998-1000
Main Authors: Wright, A.D. (University of Missouri, Columbia, MO), Sampson, M.B, Neuffer, M.G, Michalczuk, L, Slovin, J.P, Cohen, J.D
Format: Article
Language:English
Subjects:
ACIDE INDOLACETIQUE
ACIDO INDOLACETICO
BIOSINTESIS
BIOSYNTHESE
Biosynthesis
Botany
Corn
Deuterium
Embryos
Hormones
Indoles
Ions
Isotopic labeling
LOCI
LOCUS
MUTANT
MUTANTES
Mutation
Mutation (Biology)
Pericarp
Plants
Proteins
Seedlings
TRIPTOFANO
Tryptophan
TRYPTOPHANE
ZEA MAYS
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Summary:The maize mutant orange pericarp is a tryptophan auxotroph, which results from mutation of two unlinked loci of tryptophan synthase B. This mutant was used to test the hypothesis that tryptophan is the precursor to the plant hormone indole-3-acetic acid (IAA). Total IAA in aseptically grown mutant seedlings was 50 times greater than in normal seedlings. In mutant seedlings grown on media containing stable isotope-labeled precursors, IAA was more enriched than was tryptophan. No incorporation of label into IAA from tryptophan could be detected. These results establish that IAA can be produced de novo without tryptophan as an intermediate
ISSN:0036-8075
1095-9203
DOI:10.1126/science.254.5034.998