Endothelial Cells Stimulate Self-Renewal and Expand Neurogenesis of Neural Stem Cells

Neural stem cells are reported to lie in a vascular niche, but there is no direct evidence for a functional relationship between the stem cells and blood vessel component cells. We show that endothelial cells but not vascular smooth muscle cells release soluble factors that stimulate the self-renewa...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2004-05, Vol.304 (5675), p.1338-1340
Main Authors: Shen, Qin, Goderie, Susan K., Jin, Li, Karanth, Nithin, Sun, Yu, Abramova, Natalia, Vincent, Peter, Pumiglia, Kevin, Temple, Sally
Format: Article
Language:English
Subjects:
Adult stem cells
Anatomy
Anatomy & physiology
Animals
Astrocytes - cytology
Astrocytes - physiology
Biological and medical sciences
Blood vessels
Cattle
Cell Adhesion
Cell Communication
Cell Differentiation
Cell Division
Cell Line
Cell Lineage
Cell lines
Cells, Cultured
Cerebral Cortex - embryology
Clone Cells - physiology
Coculture Techniques
Development. Senescence. Regeneration. Transplantation
Embryo, Mammalian - cytology
Embryonic stem cells
Embryos
Endothelial cells
Endothelial Cells - cytology
Endothelial Cells - physiology
Endothelium, Vascular - cytology
Environmental Influences
Feeder cells
Fibroblast Growth Factor 2 - pharmacology
Fundamental and applied biological sciences. Psychology
Individualized Instruction
Mice
Muscle, Smooth, Vascular - cytology
Muscle, Smooth, Vascular - physiology
Myocytes, Smooth Muscle - cytology
Myocytes, Smooth Muscle - physiology
Neural stem cells
Neurons
Neurons - cytology
Neurons - physiology
NIH 3T3 cells
Oligodendroglia - cytology
Oligodendroglia - physiology
Signal Transduction
Statistical Analysis
Stem cells
Stem Cells - cytology
Stem Cells - physiology
Vertebrates: nervous system and sense organs
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Summary:Neural stem cells are reported to lie in a vascular niche, but there is no direct evidence for a functional relationship between the stem cells and blood vessel component cells. We show that endothelial cells but not vascular smooth muscle cells release soluble factors that stimulate the self-renewal of neural stem cells, inhibit their differentiation, and enhance their neuron production. Both embryonic and adult neural stem cells respond, allowing extensive production of both projection neuron and interneuron types in vitro. Endothelial coculture stimulates neuroepithelial cell contact, activating Notch and Hes1 to promote self-renewal. These findings identify endothelial cells as a critical component of the neural stem cell niche.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1095505