An LRR Receptor Kinase Involved in Perception of a Peptide Plant Hormone, Phytosulfokine

• Published in: Science (American Association for the Advancement of Science) 2002-05, Vol.296 (5572), p.1470-1472
• Main Authors: Matsubayashi, Yoshikatsu, Ogawa, Mari, Morita, Akiko, Sakagami, Youji
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Amino Acid Sequence; Amino acids; Base Sequence; Binding, Competitive; Biological and medical sciences; Carrots; Cell growth; Cell Line; Chromatography, Affinity; Coding; Complementary DNA; Daucus carota; Daucus carota - cytology; Daucus carota - enzymology; Daucus carota - genetics; Daucus carota - growth & development; DNA, Complementary; Economic plant physiology; Flowers & plants; Fractions; Fundamental and applied biological sciences. Psychology; Genes, Plant; Glycosylation; Growth; Growth and development; Growth regulators; Hormone receptors; Hormones; Leucine; Ligands; LRR receptor kinase; Membranes; Mesophyll cells; Metabolism; Microsomes - enzymology; Molecular Sequence Data; Molecular Weight; Peptide Hormones; Peptides; Phytosulfokine; Plant cells; Plant cells and tissues; Plant Growth Regulators; Plant physiology and development; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - isolation & purification; Plant Proteins - metabolism; Plants, Genetically Modified; Polymerase Chain Reaction; Protein kinases; Protein Structure, Tertiary; Receptors; Receptors, Cell Surface - chemistry; Receptors, Cell Surface - genetics; Receptors, Cell Surface - isolation & purification; Receptors, Cell Surface - metabolism; Repetitive Sequences, Amino Acid; Scientific Concepts; Stem cells; Totipotent stem cells
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1069607

The sulfated peptide phytosulfokine (PSK) is an intercellular signal that plays a key role in cellular dedifferentiation and proliferation in plants. Using ligand-based affinity chromatography, we purified a 120-kilodalton membrane protein, specifically interacting with PSK, from carrot microsomal fractions. The corresponding complementary DNA encodes a 1021-amino acid receptor kinase that contains extracellular leucine-rich repeats, a single transmembrane domain, and a cytoplasmic kinase domain. Overexpression of this receptor kinase in carrot cells caused enhanced callus growth in response to PSK and a substantial increase in the number of tritium-labeled PSK binding sites, suggesting that PSK and this receptor kinase act as a ligand-receptor pair.