Characterization of Lipopolysaccharide of Haemophilus influenzae

Lipopolysaccharide from strains of Haemophilus influenzae was extracted and isolated by the hot phenol-water procedure. The preparations were relatively insoluble in water but could be solubilized with surface-active agents. The preparations contained carbohydrate (30%), fatty acid (29%), and phosph...

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Bibliographic Details
Published in:The Journal of infectious diseases 1978-12, Vol.138 (6), p.719-730
Main Authors: Flesher, Alan R., Insel, Richard A.
Format: Article
Language:English
Subjects:
Animals
Antigens, Bacterial - isolation & purification
Antiserum
B lymphocytes
Bacterial Proteins - isolation & purification
Endotoxins - immunology
Fatty acids
Fatty Acids - isolation & purification
Galactose - isolation & purification
Glucosamine - isolation & purification
Glucose - isolation & purification
Haemophilus influenzae - analysis
Haemophilus influenzae type b
Heptoses
Heptoses - isolation & purification
Ketoses - isolation & purification
Lipids
Lipopolysaccharides
Lipopolysaccharides - analysis
Lipopolysaccharides - immunology
Lipopolysaccharides - isolation & purification
Male
Mice
Phosphates - isolation & purification
Polymyxins
Rabbits
Salmonella
Scientific Articles
Shwartzman Phenomenon - etiology
Solubility
Spleen cells
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Description
Summary:Lipopolysaccharide from strains of Haemophilus influenzae was extracted and isolated by the hot phenol-water procedure. The preparations were relatively insoluble in water but could be solubilized with surface-active agents. The preparations contained carbohydrate (30%), fatty acid (29%), and phosphate (4.7%); protein content was
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/138.6.719