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Characterization of Lipopolysaccharide of Haemophilus influenzae
Lipopolysaccharide from strains of Haemophilus influenzae was extracted and isolated by the hot phenol-water procedure. The preparations were relatively insoluble in water but could be solubilized with surface-active agents. The preparations contained carbohydrate (30%), fatty acid (29%), and phosph...
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Published in: | The Journal of infectious diseases 1978-12, Vol.138 (6), p.719-730 |
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container_title | The Journal of infectious diseases |
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creator | Flesher, Alan R. Insel, Richard A. |
description | Lipopolysaccharide from strains of Haemophilus influenzae was extracted and isolated by the hot phenol-water procedure. The preparations were relatively insoluble in water but could be solubilized with surface-active agents. The preparations contained carbohydrate (30%), fatty acid (29%), and phosphate (4.7%); protein content was |
doi_str_mv | 10.1093/infdis/138.6.719 |
format | article |
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The preparations were relatively insoluble in water but could be solubilized with surface-active agents. The preparations contained carbohydrate (30%), fatty acid (29%), and phosphate (4.7%); protein content was <1 %. Thin-layer chromatography, gas-liquid chromatography, and colorimetric assays detected glucose, galactose, glucosamine, heptose, and a 2-keto-3-deoxyoctonate- like molecule <1 %). Neither methylpentose nor dideoxyhexose was detected. The lipid portion was composed of fatty acids common to lipopolysaccharide of Salmonella. The preparations provoked positive dermal Shwartzman reactions and biphasic febrile responses in rabbits, responses typical of endotoxic activity. The 50% lethal dose for mice was decreased from 16.5 μg/g to 0.015 μg/g by concomitant administration of actinomycin D. The preparations were shown to be polyclonal activators of bone marrow-derived (B) cells. Limulus lysate gelation was seen with 8.0 ng of lipopolysaccharide. Preliminary hemagglutination data suggested at least three different antigenic factors associated with the lipopolysaccharide of H. influenzae type b. The H. influenzae lipopolysaccharide appeared biologically similar to that of enterobacteria but chemically different.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1093/infdis/138.6.719</identifier><identifier>PMID: 310855</identifier><language>eng</language><publisher>United States: The University of Chicago Press</publisher><subject><![CDATA[Animals ; Antigens, Bacterial - isolation & purification ; Antiserum ; B lymphocytes ; Bacterial Proteins - isolation & purification ; Endotoxins - immunology ; Fatty acids ; Fatty Acids - isolation & purification ; Galactose - isolation & purification ; Glucosamine - isolation & purification ; Glucose - isolation & purification ; Haemophilus influenzae - analysis ; Haemophilus influenzae type b ; Heptoses ; Heptoses - isolation & purification ; Ketoses - isolation & purification ; Lipids ; Lipopolysaccharides ; Lipopolysaccharides - analysis ; Lipopolysaccharides - immunology ; Lipopolysaccharides - isolation & purification ; Male ; Mice ; Phosphates - isolation & purification ; Polymyxins ; Rabbits ; Salmonella ; Scientific Articles ; Shwartzman Phenomenon - etiology ; Solubility ; Spleen cells]]></subject><ispartof>The Journal of infectious diseases, 1978-12, Vol.138 (6), p.719-730</ispartof><rights>Copyright 1978 The University of Chicago</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c268t-8f806cdd4c7e294b005c84d8fc93c503bd126a4c4cebb02050ada2847ab83a173</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/310855$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Flesher, Alan R.</creatorcontrib><creatorcontrib>Insel, Richard A.</creatorcontrib><title>Characterization of Lipopolysaccharide of Haemophilus influenzae</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>Lipopolysaccharide from strains of Haemophilus influenzae was extracted and isolated by the hot phenol-water procedure. The preparations were relatively insoluble in water but could be solubilized with surface-active agents. The preparations contained carbohydrate (30%), fatty acid (29%), and phosphate (4.7%); protein content was <1 %. Thin-layer chromatography, gas-liquid chromatography, and colorimetric assays detected glucose, galactose, glucosamine, heptose, and a 2-keto-3-deoxyoctonate- like molecule <1 %). Neither methylpentose nor dideoxyhexose was detected. The lipid portion was composed of fatty acids common to lipopolysaccharide of Salmonella. The preparations provoked positive dermal Shwartzman reactions and biphasic febrile responses in rabbits, responses typical of endotoxic activity. The 50% lethal dose for mice was decreased from 16.5 μg/g to 0.015 μg/g by concomitant administration of actinomycin D. The preparations were shown to be polyclonal activators of bone marrow-derived (B) cells. Limulus lysate gelation was seen with 8.0 ng of lipopolysaccharide. Preliminary hemagglutination data suggested at least three different antigenic factors associated with the lipopolysaccharide of H. influenzae type b. The H. influenzae lipopolysaccharide appeared biologically similar to that of enterobacteria but chemically different.</description><subject>Animals</subject><subject>Antigens, Bacterial - isolation & purification</subject><subject>Antiserum</subject><subject>B lymphocytes</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>Endotoxins - immunology</subject><subject>Fatty acids</subject><subject>Fatty Acids - isolation & purification</subject><subject>Galactose - isolation & purification</subject><subject>Glucosamine - isolation & purification</subject><subject>Glucose - isolation & purification</subject><subject>Haemophilus influenzae - analysis</subject><subject>Haemophilus influenzae type b</subject><subject>Heptoses</subject><subject>Heptoses - isolation & purification</subject><subject>Ketoses - isolation & purification</subject><subject>Lipids</subject><subject>Lipopolysaccharides</subject><subject>Lipopolysaccharides - analysis</subject><subject>Lipopolysaccharides - immunology</subject><subject>Lipopolysaccharides - isolation & purification</subject><subject>Male</subject><subject>Mice</subject><subject>Phosphates - isolation & purification</subject><subject>Polymyxins</subject><subject>Rabbits</subject><subject>Salmonella</subject><subject>Scientific Articles</subject><subject>Shwartzman Phenomenon - etiology</subject><subject>Solubility</subject><subject>Spleen cells</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><recordid>eNpFkD1PwzAQhi3EV_nYGUDqxJb2HDu2s0EroEgVDBSEWCzHcYQhrYOdSJRfj6tUMJ10z917ugehMwwjDDkZ21VV2jDGRIzYiON8Bw1wRnjCGCa7aACQpgkWeX6IjkL4AABKGD9A-wSDyLIBupq-K690a7z9Ua11q6GrhnPbuMbV66C0jtiWZtOdKbN0zbutuzCMZ-vOrH6UOUF7laqDOd3WY_R8e7OYzpL549399Hqe6JSJNhGVAKbLkmpu0pwWAJkWtBSVzonOgBQlTpmimmpTFJBCBqpUqaBcFYIozMkxuuxzG---OhNaubRBm7pWK-O6IDklJI1Px0HoB7V3IXhTycbbpfJriUFunMnemYzOJJPRWVw532Z3xdKUfwu9pIgvevwRWuf_KcQ0IJuLSc9taM33H1f-UzJOeCZnr2_y7WUxofzhSU7ILwu0gpg</recordid><startdate>197812</startdate><enddate>197812</enddate><creator>Flesher, Alan R.</creator><creator>Insel, Richard A.</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197812</creationdate><title>Characterization of Lipopolysaccharide of Haemophilus influenzae</title><author>Flesher, Alan R. ; Insel, Richard A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c268t-8f806cdd4c7e294b005c84d8fc93c503bd126a4c4cebb02050ada2847ab83a173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>Animals</topic><topic>Antigens, Bacterial - isolation & purification</topic><topic>Antiserum</topic><topic>B lymphocytes</topic><topic>Bacterial Proteins - isolation & purification</topic><topic>Endotoxins - immunology</topic><topic>Fatty acids</topic><topic>Fatty Acids - isolation & purification</topic><topic>Galactose - isolation & purification</topic><topic>Glucosamine - isolation & purification</topic><topic>Glucose - isolation & purification</topic><topic>Haemophilus influenzae - analysis</topic><topic>Haemophilus influenzae type b</topic><topic>Heptoses</topic><topic>Heptoses - isolation & purification</topic><topic>Ketoses - isolation & purification</topic><topic>Lipids</topic><topic>Lipopolysaccharides</topic><topic>Lipopolysaccharides - analysis</topic><topic>Lipopolysaccharides - immunology</topic><topic>Lipopolysaccharides - isolation & purification</topic><topic>Male</topic><topic>Mice</topic><topic>Phosphates - isolation & purification</topic><topic>Polymyxins</topic><topic>Rabbits</topic><topic>Salmonella</topic><topic>Scientific Articles</topic><topic>Shwartzman Phenomenon - etiology</topic><topic>Solubility</topic><topic>Spleen cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Flesher, Alan R.</creatorcontrib><creatorcontrib>Insel, Richard A.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Flesher, Alan R.</au><au>Insel, Richard A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of Lipopolysaccharide of Haemophilus influenzae</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>1978-12</date><risdate>1978</risdate><volume>138</volume><issue>6</issue><spage>719</spage><epage>730</epage><pages>719-730</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><abstract>Lipopolysaccharide from strains of Haemophilus influenzae was extracted and isolated by the hot phenol-water procedure. The preparations were relatively insoluble in water but could be solubilized with surface-active agents. The preparations contained carbohydrate (30%), fatty acid (29%), and phosphate (4.7%); protein content was <1 %. Thin-layer chromatography, gas-liquid chromatography, and colorimetric assays detected glucose, galactose, glucosamine, heptose, and a 2-keto-3-deoxyoctonate- like molecule <1 %). Neither methylpentose nor dideoxyhexose was detected. The lipid portion was composed of fatty acids common to lipopolysaccharide of Salmonella. The preparations provoked positive dermal Shwartzman reactions and biphasic febrile responses in rabbits, responses typical of endotoxic activity. The 50% lethal dose for mice was decreased from 16.5 μg/g to 0.015 μg/g by concomitant administration of actinomycin D. The preparations were shown to be polyclonal activators of bone marrow-derived (B) cells. Limulus lysate gelation was seen with 8.0 ng of lipopolysaccharide. Preliminary hemagglutination data suggested at least three different antigenic factors associated with the lipopolysaccharide of H. influenzae type b. The H. influenzae lipopolysaccharide appeared biologically similar to that of enterobacteria but chemically different.</abstract><cop>United States</cop><pub>The University of Chicago Press</pub><pmid>310855</pmid><doi>10.1093/infdis/138.6.719</doi><tpages>12</tpages></addata></record> |
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source | Oxford University Press:Jisc Collections:Oxford Journal Archive: Access period 2024-2025 |
subjects | Animals Antigens, Bacterial - isolation & purification Antiserum B lymphocytes Bacterial Proteins - isolation & purification Endotoxins - immunology Fatty acids Fatty Acids - isolation & purification Galactose - isolation & purification Glucosamine - isolation & purification Glucose - isolation & purification Haemophilus influenzae - analysis Haemophilus influenzae type b Heptoses Heptoses - isolation & purification Ketoses - isolation & purification Lipids Lipopolysaccharides Lipopolysaccharides - analysis Lipopolysaccharides - immunology Lipopolysaccharides - isolation & purification Male Mice Phosphates - isolation & purification Polymyxins Rabbits Salmonella Scientific Articles Shwartzman Phenomenon - etiology Solubility Spleen cells |
title | Characterization of Lipopolysaccharide of Haemophilus influenzae |
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