Enhanced production of manganese peroxidase from immobilized Phanerochaete chrysosporium due to the increased autolysis of chlamydospore-like cells

The autolysis of chlamydospore-like cells in Phanerochaete chrysosporium immobilized in polyurethane foam correlated with the production of manganese peroxidase (MnP). The maximum specific activity of MnP was 1055 U g dry mycelium(-1) in the immobilized culture, compared with 260 U g dry mycelium(-1...

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Bibliographic Details
Published in:Biotechnology letters 2005-04, Vol.27 (7), p.477-481
Main Authors: CHUNG, Eunyong, OH, Junse, HWANG, Sangpill, AHN, Ik-Sung, YEO JOON YOON
Format: Article
Language:English
Subjects:
Biodegradation
Biological and medical sciences
Biomass
Biotechnology
Cells, Immobilized
Fermentation
Fundamental and applied biological sciences. Psychology
Manganese
Microbiology
Microscopy, Phase-Contrast
Mycelium - cytology
Peroxidases - biosynthesis
Peroxidases - isolation & purification
Phanerochaete - metabolism
Phanerochaete chrysosporium
Polyurethanes
Spores, Fungal - cytology
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Summary:The autolysis of chlamydospore-like cells in Phanerochaete chrysosporium immobilized in polyurethane foam correlated with the production of manganese peroxidase (MnP). The maximum specific activity of MnP was 1055 U g dry mycelium(-1) in the immobilized culture, compared with 260 U g dry mycelium(-1) in the submerged culture. Scattered mycelial pellets were formed in the immobilized culture in which almost all of the chlamydospore-like cells were subject to autolysis. However, highly crowded pellets were formed in the free culture, in which only the chlamydospore-like cells in the exterior were subject to autolysis. We propose that the enhanced production of MnP in immobilized cultures of P. chrysosporium is due to increased autolysis of the chlamydospore-like cells.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-005-2224-9